期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2213373119/-/DCSupplemental
关键词
biomimetic nanoparticles; photoacoustic; rheumatoid arthritis; targeted imaging; small interfering; RNA
资金
- National Natural Science Foundation of China [82172008]
- National Key R&D Program of China [2020YFA0908800]
- CAS Key Laboratory of Health Informatics [2011DP173015]
- Guangdong Provincial Key Laboratory of Biomedical Optical Imaging [2020B121201010]
- Guangdong Basic and Applied Basic Research Foundation [2020A1515010978]
- Robert A. Welch Foundation [F-0018]
This study presents a new strategy for the diagnosis, treatment, and monitoring of rheumatoid arthritis (RA) by using macrophage biomimetic nanoparticles and small interfering RNA (siRNA) to regulate the RA microenvironment. The siRNA is able to silence proinflammatory cytokine expression, while the nanoparticles scavenge reactive oxygen species (ROS). Near-infrared photoacoustic (PA) imaging allows real-time monitoring and evaluation of the treatment. Overall, this approach shows potential for improving the management of RA.
The high level of reactive oxygen species (ROS) in the rheumatoid arthritis (RA) microenvironment (RAM) and its persistent inflammatory nature can promote damage to joints, bones, and the synovium. Targeting strategies that integrate effective RAM regulation with imaging-based monitoring could lead to improvements in the diagnosis and treatment of RA. Here, we report the combined use of small interfering RNAs (siRNAsT/I) and Prussian blue nanoparticles (PBNPs) to silence the expression of proinflammatory cytokines TNFa/IL-6 and scavenge the ROS associated with RAM. To enhance the in vitro and in vivo biological stability, biocompatibility, and targeting capability of the siRNAsT/I and PBNPs, macrophage membrane vesicles were used to prepare biomimetic nanoparticles, M@PsiRNAsT/I. The resulting constructs were found to suppress tumor necrosis factor-a/ interleukin-6 expression and overcome the hypoxic nature of RAM, thus alleviating RA-induced joint damage in a mouse model. The M@P-siRNAsT/I of this study could be monitored via near-infrared photoacoustic (PA) imaging. Moreover, multispectral PA imaging without the need for labeling permitted the real-time evaluation of M@PsiRNAsT/I as a putative RA treatment. Clinical microcomputed tomography and histological analysis confirmed the effectiveness of the treatment. We thus suggest that macrophage-biomimetic M@P-siRNAsT/I and their analogs assisted by PA imaging could provide a new strategy for RA diagnosis, treatment, and monitoring.
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