Implementing molecular tuberculosis diagnostic methods in limited-resource and high-burden countries

Article Multidisciplinary Sciences

Implementation of a COVID-19 Genomic Surveillance Regional Network for Latin America and Caribbean region

Juliana Almeida Leite et al.

Summary: The timely release of SARS-CoV-2 genomic sequences is crucial for the response to the pandemic. The Pan American Health Organization has collaborated with countries to establish a network aimed at strengthening the timely generation of SARS-CoV-2 genomic data in Latin America and the Caribbean. Genomic surveillance provides important data for guiding public health decisions and enhancing laboratory response capacity.

PLOS ONE (2022)

添加到收藏夹

Article Infectious Diseases

The 2021 WHO catalogue of Mycobacterium tuberculosis complex mutations associated with drug resistance: a genotypic analysis

Timothy M. Walker et al.

Summary: This study aimed to generate a WHO-endorsed catalogue of mutations for drug resistance prediction in Mycobacterium tuberculosis complex (MTBC) and provide a global standard for interpreting molecular information. The research analyzed MTBC isolates from 45 countries and identified mutations associated with resistance to different antituberculosis drugs. The findings can encourage the implementation of molecular diagnostics by national tuberculosis programs.

LANCET MICROBE (2022)

添加到收藏夹

Article Biochemical Research Methods

Capacity building for whole genome sequencing of Mycobacterium tuberculosis and bioinformatics in high TB burden countries

Emmanuel Riviere et al.

Summary: Asia and Africa are underrepresented in Mtb WGS research compared to their TB burden, leading to adaptations in training, mentoring, and infrastructure building. Utilizing case studies created a safe space for students to voice and defend their opinions. Ensuring quality DNA extraction, biosafety level 3 and general laboratory skill training, commercial DNA extraction kits, and a combination of training in a highly equipped laboratory and local setting were added to the program.

BRIEFINGS IN BIOINFORMATICS (2021)

添加到收藏夹

Review Biotechnology & Applied Microbiology

Nanopore sequencing technology, bioinformatics and applications

Yunhao Wang et al.

Summary: Advancements in nanopore technologies for sequencing long DNA and RNA molecules have greatly improved accuracy, read length, and throughput. The field of nanopore sequencing continues to offer opportunities for enhancing data quality and analytical approaches through the development of new technologies tailored to specific applications.

NATURE BIOTECHNOLOGY (2021)

添加到收藏夹

Article Multidisciplinary Sciences