DAPT Alleviates Cardiac Dysfunction in Psoriasis by Inhibiting Angiogenesis Through Regulating the Notch/DLL4 Pathway

This study aims to explore the effect of DAPT on cardiac dysfunction in psoriasis. The psoriasis model was established in mice by smeared with 50 mg/cm(2) imiquimod (IMQ), followed by administration of 3, 10, and 30 mg/kg DAPT, respectively. The elevated thickness of epidermal layers observed IMQ-treated mice was greatly reduced by DAPT, accompanied by a downregulation of VEGF and CD34. Furthermore, the activated Notch/DLL4 pathway in IMQ-treated mice was repressed by DAPT. The increased value of LVIDd, LVIDs, and heart index, as well as the declined EF, were dramatically reversed by DAPT, accompanied by repaired ultrastructure in myocardial cells and reduced number of collagen fibers observed in cardiac tissues. In addition, the activated Notch/DLL4 pathway in cardiac tissues of IMQ-treated mice was inhibited IP: 2038 10920 On: Fr, 31 Mar 2023 08:03:21 by DAPT. A co-cultural system was established between HUVECs and HaCaT cells, followed by incubating with 2.5 mu M Copyright: Amer can Scientific Pub ishers DAPT, 5 mu M DAPT, and 10 mu M DAPT for 48 hours, rspctivel. A dramtically declined cell viability, increased apoptotic Delivered by Ingenta rate, and suppressed tube formation capacity were observed in HUVECs after the treatment of DAPT, accompanied by an inhibition of Notch/DII4 signaling. Collectively, DAPT alleviated cardiac dysfunction in psoriasis by inhibiting angiogenesis through regulating the Notch/DLL4 pathway.

Automated summary

This study aimed to investigate the effect of dual antiplatelet therapy (DAPT) on cardiac dysfunction in psoriasis. The results showed that DAPT alleviated cardiac dysfunction caused by psoriasis by inhibiting angiogenesis and regulating the Notch/DLL4 pathway.