Research on Mechanism of miR-488-5p Carried with Magnetic Carbon Nanotubes in Restraining Lymphatic Metastasis in Cervical Cancer Through Induction of mTOR/P70S6K Signaling Pathway

This study intends to discuss mechanism of miR-488-5p carried with magnetic carbon nanotubes (MAGCNTs) in restraining lymphatic metastasis in cervical cancer through induction of mTOR/P70S6K signaling pathway. Fifty female rats were randomly divided into five sets, which included control set, miR-488-5p set, empty carrier set, set of miR-488-5p carrier and inhibitor set. The MAGCNTs were prepared and characterized. Condition and quantity of lymphatic metastasis in cervical cancer, miR-488-5p expression and protein expression of factors related with mTOR/P70S6K signaling transduction pathway were observed. Quantity of lymphatic metastasis in the control set was highest, while quantity of lymphatic metastasis in the set of miR-488-5p and carrier were lowest. Expressions of miR-488-5p in of the miR-488-5p and carrier sets were highest and lowest in the control set, and mTOR/P70S6K expression was reversed. miR-488-5p was carried with multi-walled carbon nanotubes (MWCNTs) and entered into cells more rapidly. There was over expression of miR-488-5p and activity of mTOR/P70S6K signaling pathway was restrained. The information conduction was slowed down, and therefore, the lymphatic metastasis in the cervical cancer was restrained.

Automated summary

This study demonstrates the mechanism of miR-488-5p carried by magnetic carbon nanotubes (MAGCNTs) in restraining lymphatic metastasis in cervical cancer through induction of mTOR/P70S6K signaling pathway. The results show that quantity of lymphatic metastasis was lowest in the miR-488-5p carrier group, with highest expression of miR-488-5p and inhibited activity of the mTOR/P70S6K signaling pathway.