Exploitation of Yeasts with Probiotic Traits for Kefir Production: Effectiveness of the Microbial Consortium

Kefir is a fermented milk made by beneficial lactic acid bacteria and yeasts inoculated as grains or free cultures. In this work, five yeast strains with probiotic aptitudes belonging to Candida zeylanoides, Yarrowia lipolytica, Kluyveromyces lactis, and Debaryomyces hansenii species were assessed in a defined consortium, in co-culture with a commercial strain of Lactobacillus casei, in order to evaluate the yeasts' fermentation performance during kefir production, using different milks. The concentration of each yeast was modulated to obtain a stable consortium that was not negatively affected by the bacteria. Furthermore, all yeasts remained viable for five weeks at 4 degrees C, reaching about 8.00 Log CFU in 150 mL of kefir, a volume corresponding to a pot of a commercial product. The yeasts consortium showed a suitable fermentation performance in all milks, conferring peculiar and distinctive analytical and aromatic properties to the kefirs, confirmed by a pleasant taste. Overall, the panel test revealed that the cow's and sheep's kefir were more appreciated than the others; this evaluation was supported by a distinctive fermentation by-products' content that positively influences the final aroma, conferring to the kefir exalted taste and complexity. These results allow us to propose the yeasts consortium as a versatile and promising multistarter candidate able to affect industrial kefir with both recognizable organoleptic properties and probiotic aptitudes.

Automated summary

This study evaluated the fermentation performance of five yeast strains with probiotic aptitudes in co-culture with a commercial strain of Lactobacillus casei for kefir production using different milks. The results showed that the yeast consortium exhibited suitable fermentation performance and conferred peculiar and distinctive properties to the kefirs, resulting in a pleasant taste. Cow's and sheep's kefir were preferred by the panel test, which was supported by distinctive fermentation by-products' content positively influencing the final aroma.