期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 4, 期 14, 页码 2407-2411出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6tb00426a
关键词
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资金
- Hong Kong Baptist University [FRG2/14-15/004, FRG2/15-16/002]
- Health and Medical Research Fund [HMRF/14130522]
- Research Grants Council [HKBU/201811, HKBU/204612, HKBU/201913]
- French Agence Nationale de la Recherche/Research Grants Council [A-HKBU201/12, ANR-12-IS07-0001]
- National Natural Science Foundation of China [21575121]
- Guangdong Province Natural Science Foundation [2015A030313816]
- Hong Kong Baptist University Century Club [RC-IRMS/14-15/06]
- Science and Technology Development Fund, Macao SAR [098/2014/A2]
- University of Macau [MYRG091(Y3-L2)-ICMS12-LCH, MYRG2015-00137-ICMSQRCM, MRG023/LCH/2013/ICMS, MRG044/LCH/2015/ICMS]
A novel Ir(III) complex 1 displays high selectivity for the G-quadruplex, and was used to establish a label-free G-quadruplex-based detection platform for lysozyme in buffer. In this study, we employed a special feature of most G-quadruplex probes that they do not respond towards the TBA G-quadruplex. A duplex DNA with a TBA G-quadruplex tail was designed for the detection of lysozyme. The presence of lysozyme will induce duplex dissociation and release the hybridized c-kit87 G-quadruplex, which would be recognized by the Ir(III) complex to generate a strong luminescence response. Common duplex DNA designs lacking a TBA G-quadruplex tail typically contain long complementary DNA regions that may be too stable to be dissociated, thus decreasing sensitivity. We anticipate that the presence of a TBA tail in the DNA duplex may destabilize the duplex structure, allowing the aptamer to be more easily dissociated and bind to the lysozyme, thereby increasing the sensitivity of the lysozyme detection platform. To our knowledge, this is the first example of the use of the TBA G-quadruplex to improve sensitivity through fine-tuning duplex stability. We believe that this approach may be further employed in sensing platforms for other targets. This assay exhibited a linear response for lysozyme within the concentration range of 2-50 nM (R-2 = 0.9904), and the limit of detection for lysozyme was 2 nM. Moreover, this platform exhibited a potential use for biological sample analysis.
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