期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 4, 期 7, 页码 1270-1275出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5tb02195b
关键词
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资金
- National Nature Science Foundation of China [21275087, 21422504, 21505081]
- Natural Science Foundation of Shandong Province of China [ZR2012BM008, JQ201406]
- Taishan Scholar Program of Shandong Province, China
In this work, we develop a facile one-step strategy for rapidly preparing dual ligand co-functionalized fluorescent gold nanoclusters (Au NCs) and establish a turn on approach for the rapid and selective sensing of glutathione (GSH) in aqueous solution and living cells. The as-prepared Au NCs exhibited orange red fluorescence (lambda(em) = 608 nm), a long lifetime (5.62 mu s), a large stokes shift (>300 nm), and considerable stability and were systematically characterized by using fluorescence spectroscopy, high-resolution transmission electron microscopy (HRTEM), dynamic light scattering (DLS), X-ray photoelectron spectroscopy (XPS), fluorescence lifetime, infrared (IR) spectroscopy and ultraviolet absorption spectroscopy. Based on the fluorescence recovery induced by competitive coordination with Cu2+ between Au NCs and GSH, the present turn on approach offers a high sensitivity and excellent selectivity toward GSH detection with a detection limit of 9.7 nM. More importantly, this turn on approach could also be successfully applied for visualizing and monitoring the changes in the intracellular GSH level in Hep G2 cells, providing available potential for diagnostic applications.
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