期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 4, 期 20, 页码 3544-3554出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5tb02745d
关键词
-
资金
- office of Naval Research Young National Investigator Award
- National Institutes of Health [EB012597, AR057837, DE021468, HL099073, AI105024, AR063745]
- Presidential Early Career Award for Scientists and Engineers (PECASE)
- German Heart Foundation, Frankfurt, Germany
- URB (University Research Board) grant from American University of Beirut (AUB)
- Faculty of Medicine, AUB
- CNRS (National Council for Scientific Research) grant
- SENESCYT, Quito, Ecuador
- National Strategic Technologies and Innovation Program of King Abdulaziz City for Science and Technology (KACST) [11-NAN1544-03]
- National Institute of General Medical Sciences, NIH [P20GM103638-04]
- University of Kansas
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL099073] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R56AI105024] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR057837, R56AR063745] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB012597] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH [R01DE021468] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P20GM103638] Funding Source: NIH RePORTER
The ability to modulate stem cell differentiation in a three dimensional (3D) microenvironment for bone tissue engineering in the absence of exogenous pharmaceutical agents such as bone morphogenic protein (BMP-2) remains a challenge. In this study, we introduce extracellular matrix (ECM)-mimicking nanocomposite hydrogels to induce the osteogenic differentiation of human mesenchymal stem cells (hMSCs) for bone regeneration in the absence of any osteoinductive factors. In particular, we have reinforced a photocrosslinkable collagen-based matrix (gelatin methacryloyl, GelMA) using disk-shaped nanosilicates (nSi), a new class of two-dimensional (2D) nanomaterials. We show that nanoengineered hydrogels supported the migration and proliferation of encapsulated hMSCs, with no signs of cell apoptosis or inflammatory cytokine responses. The addition of nSi significantly enhances the osteogenic differentiation of encapsulated hMSCs as evident from the increase in alkaline phosphates (ALP) activity and the deposition of a biomineralized matrix compared to GelMA. We also show that microfabricated nanoengineered microgels can be used to pattern and control cellular behaviour. Furthermore, we demonstrate that nanoengineered hydrogel have high biocompatibility as determined by in vivo experiments using an immunocompetent rat model. Specifically, the hydrogels showed minimum localized immune responses, indicating their ability for tissue engineering applications. Overall, we showed the ability of nanoengineered hydrogels loaded with 2D nanosilicates for the osteogenic differentiation of stem cells in vitro, in the absence of any growth factors such as BMP-2. Our in vivo studies show high biocompatibility of nanocomposites and show the potential for growth factor free bone regeneration.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据