期刊
NANOSCALE
卷 14, 期 39, 页码 14695-14710出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2nr03611h
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- Agence Nationale de la Recherche (ANR) [ANR-18-CE34-0005-01]
- Agence Nationale de Securite Sanitaire de l'Alimentation, de l'Environnement et du Travail (ANSES) [EST-2015/1/005]
- University of Strasbourg
- CNRS
- Inserm [ANR-10IDEX-0002, ANR-20-SFRI-0012]
- French Proteomic Infrastructure (ProFI) [ANR-10-INBS-08-03]
This study reported the first quantitative proteomic analysis of the protein corona formed on carbon dots (CDs) with different surface charge properties, showing that the surface charge properties of CDs influence protein corona composition, which in turn affects CD uptake and viability in macrophages.
Carbon dots are emerging nanoparticles (NPs) with tremendous applications, especially in the biomedical field. Herein is reported the first quantitative proteomic analysis of the protein corona formed on CDs with different surface charge properties. Four CDs were synthesized from citric acid and various amine group-containing passivation reagents, resulting in cationic NPs with increasing zeta (zeta)-potential and density of positive charges. After CD contact with serum, we show that protein corona identity is influenced by CD surface charge properties, which in turn impacts CD uptake and viability loss in macrophages. In particular, CDs with high zeta-potential (>+30 mV) and charge density (>2 mu mol mg(-1)) are the most highly internalized, and their cell uptake is strongly correlated with a corona enriched in vitronectin, fibulin, fetuin, adiponectin and alpha-glycoprotein. On the contrary, CDs with a lower zeta-potential (+11 mV) and charge density (0.01 mu mol mg(-1)) are poorly internalized, while having a corona with a very different protein signature characterized by a high abundance of apolipoproteins (APOA1, APOB and APOC), albumin and hemoglobin. These data illustrate how corona characterization may contribute to a better understanding of CD cellular fate and biological effects, and provide useful information for the development of CDs for biomedical applications.
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