期刊
NANO RESEARCH
卷 16, 期 2, 页码 3010-3020出版社
TSINGHUA UNIV PRESS
DOI: 10.1007/s12274-022-4884-5
关键词
near-infrared probe conjugates; non-covalent albumin-binding; fluorescence imaging; sentinel lymph node metastasis illumination; primary tumor diagnosis
This article reports three novel IR820-fatty acid conjugates for diagnosis of primary tumors and lymphatic metastases. The conjugates can bind to albumin in vivo, enhancing fluorescence quantum yield, and show a large Stokes shift for improved imaging resolution. Among them, IR-PA demonstrates better fluorescence quantum yield, photostability, and imaging resolution than other conjugates. These findings provide a self-adaptive albumin-binding near-infrared probe conjugate for accurate diagnosis of primary tumors and lymphatic metastases.
Tumor sentinel lymph node (SLN) metastasis plays a vital role in tumor staging and therapeutic decision-making process. However, precise diagnosis of primary tumors and lymphatic metastases is still hindered by low imaging resolution and poor photostability of fluorescent probes. Herein, we report three novel IR820-fatty acid (FA) conjugates (IR-OA, IR-LA, and IR-PA) for precise lymphatic metastasis illumination and primary tumor diagnosis. The IR-FA conjugates are able to non-covalently bound to albumin in vivo, and the fluorescence quantum yield is significantly enhanced after incubation with bovine serum albumin (BSA) in vitro. Moreover, the BSA-IR-FA conjugates display large Stokes shift (> 120 nm), dramatically improving in vivo imaging resolution. Among them, IR-PA demonstrates distinct advantage over IR-OA, IR-LA, and IR-maleimide (MAL) (fluorescent probe previously reported by our group) in terms of fluorescence quantum yield, photostability, and imaging resolution. As a result, IR-PA exhibits satisfactory imaging results with high fluorescence intensity and imaging resolution in sentinel lymph node metastasis illumination and primary tumor location. Our findings provide a self-adaptive albumin-binding near-infrared probe conjugate for accurate diagnosis of primary tumors and lymphatic metastases.
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