期刊
CELLULAR & MOLECULAR IMMUNOLOGY
卷 20, 期 1, 页码 38-50出版社
CHIN SOCIETY IMMUNOLOGY
DOI: 10.1038/s41423-022-00946-2
关键词
Alveolar macrophages; Chronic obstructive pulmonary diseases; Surfactant protein D; Lipid metabolism; Ozone; Cigarettes
类别
Increased levels of SP-D and FMs are found in oxidative stress conditions and/or COPD patients. Our study reveals the crucial roles of SP-D in airway inflammation and emphysematous changes, and shows that rfhSP-D can reverse these changes.
Increased levels of surfactant protein D (SP-D) and lipid-laden foamy macrophages (FMs) are frequently found under oxidative stress conditions and/or in patients with chronic obstructive pulmonary disease (COPD) who are also chronically exposed to cigarette smoke (CS). However, the roles and molecular mechanisms of SP-D and FMs in COPD have not yet been determined. In this study, increased levels of SP-D were found in the bronchoalveolar lavage fluid (BALF) and sera of ozone- and CS-exposed mice. Furthermore, SP-D-knockout mice showed increased lipid-laden FMs and airway inflammation caused by ozone and CS exposure, similar to that exhibited by our study cohort of chronic smokers and COPD patients. We also showed that an exogenous recombinant fragment of human SP-D (rfhSP-D) prevented the formation of oxidized low-density lipoprotein (oxLDL)-induced FMs in vitro and reversed the airway inflammation and emphysematous changes caused by oxidative stress and CS exposure in vivo. SP-D upregulated bone marrow-derived macrophage (BMDM) expression of genes involved in countering the oxidative stress and lipid metabolism perturbations induced by CS and oxLDL. Our study demonstrates the crucial roles of SP-D in the lipid homeostasis of dysfunctional alveolar macrophages caused by ozone and CS exposure in experimental mouse emphysema, which may provide a novel opportunity for the clinical application of SP-D in patients with COPD.
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