期刊
JOURNAL OF THE AMERICAN HEART ASSOCIATION
卷 5, 期 1, 页码 -出版社
WILEY
DOI: 10.1161/JAHA.115.002856
关键词
angiogenesis; cardiac stem cells; exosomes; miRNA profiling; proliferation
资金
- National Natural Science Foundation of China [30972696, 81400199]
- Suzhou Municipal Science and Technology Project of China [SYS201414]
Background-Exosomes derived from mesenchymal stem cells (MSCs) were proved to boost cell proliferation and angiogenic potency. We explored whether cardiac stem cells (CSCs) preconditioned with MSC exosomes could survive and function better in a myocardial infarction model. Methods and Results-DiI-labeled exosomes were internalized with CSCs. They stimulated proliferation, migration, and angiotube formation of CSCs in a dose-dependent manner. In a rat myocardial infarction model, MSC exosome-preconditioned CSCs had significantly better survival, enhanced capillary density, reduced cardiac fibrosis, and restored long-term cardiac function. MicroRNA profiling analysis revealed that a set of microRNAs were significantly changed in CSCs after MSC exosome treatment. Conclusions-Pretreatment of CSCs with MSC exosomes provided a promising strategy to improve survival and angiogenic potency of CSCs.
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