A sea urchin-shaped nanozyme mediated dual-mode immunoassay nanoplatform for sensitive point-of-care testing histamine in food samples

Rapid detection of histamine remains a challenge due to the complexity of food matrices. Based on the high peroxidase-like activity of sea urchin-shaped Pt@Au NPs (SU-Pt@Au NPs), a novel dual-mode nanoplatform is developed for the sensitive detection of histamine utilizing an indirect competitive enzyme-linked immunosorbent assay. According to the colorimetric-based UV-vis nanoplatform, histamine is sensitively detected with a liner range from 0.5 to 100 ng/mL and a limit of detection (LOD) as low as 0.3 ng/mL. Then, a smartphoneloaded color picker APP can intelligently detect histamine in point-of-care testing (POCT) based on the R/B ratio of the color channels, with a detection range of 0.5 to 1000 ng/mL and a LOD as low as 0.15 ng/mL, significantly expanding the detection range. Such an easy-to-use and sensitive detection system is employed to quantify histamine in Pacific saury, crab, and pork samples, indicating outstanding application potential in protein-rich meat food safety.

Automated summary

A novel dual-mode nanoplatform based on sea urchin-shaped Pt@Au NPs with high peroxidase-like activity is developed for sensitive detection of histamine. The colorimetric-based UV-vis nanoplatform allows for sensitive detection of histamine with a linear range of 0.5-100 ng/mL and a low limit of detection (LOD) of 0.3 ng/mL. Additionally, a smartphone-loaded color picker APP can intelligently detect histamine based on the R/B ratio in the color channels, achieving a detection range of 0.5-1000 ng/mL and a LOD as low as 0.15 ng/mL, significantly expanding the detection range. This easy-to-use and sensitive detection system shows great potential in ensuring the safety of protein-rich meat food.