期刊
MICROBIOLOGY SPECTRUM
卷 -, 期 -, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/spectrum.01963-23
关键词
bovine herpesvirus 1; phospholipase C-& gamma;1; Golgi apparatus; gD
类别
Phospholipase C gamma 1 (PLC-gamma 1) plays an important role in the replication process of bovine herpesvirus 1 (BoHV-1), specifically in the Golgi apparatus.
Phospholipase C gamma 1 (PLC-gamma 1) may locate at distinct subcellular locations, such as cytosol, plasma membrane, and nucleus for varied biological functions. Bovine herpesvirus 1 (BoHV-1) productive infection activates PLC-gamma 1 signaling, as demonstrated by increased protein levels of phosphorylated-PLC-.1 at Ser1248 [p-PLC-.1(S1248)], which benefits virus productive infection. Here, for the first time, we reported that Golgi apparatus also contains activated p-PLC-gamma 1(S1248). And BoHV-1 productive infection at later stages (24 hpi) increased the accumulation of p-PLC.1(S1248) in the Golgi apparatus, where p-PLC-gamma 1(S1248) forms highlighted puncta observed via a confocal microscope. Coimmunoprecipitation studies demonstrated that the Golgi p-PLC-gamma 1(S1248) is specifically associated with the viral protein gD but not gC. In addition, we found that p-PLC-gamma 1(S1248) is consistently associated with both the plasma membrane-associated virions and the released virions. When the virus-infected cells were treated with PLC-gamma 1-specific inhibitor, U73122, for a short duration of 4 hours prior to the endpoint of virus infection, we found that the viral protein gD was trapped in the Golgi apparatus, suggesting that the PLC-gamma 1 signaling may facilitate traffickingtraffickingtraffickingof progeny virions out of this organelle. These findings provide a novel insight into the interplay between PLC-gamma 1 signaling and BoHV-1 replication.
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