期刊
INTERNATIONAL JOURNAL OF GENOMICS
卷 2016, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2016/5078796
关键词
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Genome editing with engineered nucleases enabling site-directed sequencemodifications bears a great potential for advanced plant breeding and crop protection. Remarkably, the RNA-guided endonuclease technology (RGEN) based on the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) is an extremely powerful and easy tool that revolutionizes both basic research and plant breeding. Here, we review the major technical advances and recent applications of the CRISPR-Cas9 system for manipulation of model and crop plant genomes. We also discuss the future prospects of this technology in molecular plant breeding.
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