Label-free DNA quantification using isothermal amplification on an exposed core optical fiber microfluidic platform

Article Zoology

An isothermal enzymatic recombinase amplification (ERA) assay for rapid and accurate detection of Enterocytozoon hepatopenaei infection in shrimp

Jiaobing Li et al.

Summary: In this study, a rapid and sensitive detection method for the prawn parasitic disease EHP was developed and evaluated. The EHP-ERA assay could diagnose EHP within 20 minutes at 42 degrees C, with a detection limit of 10 copies/mu L.

JOURNAL OF INVERTEBRATE PATHOLOGY (2023)

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Article Chemistry, Analytical

A facile enzyme-assisted multiple recycling amplification strategy for ultrasensitive fluorescence detection of HIV-1 DNA

Kexin Ding et al.

Summary: We developed an enzyme-assisted multiple signal amplification reaction for target nucleic acid detection. This strategy involves utilizing the target nucleic acid as triggers, along with specific primers, DNA polymerase, and DNA nicking endonuclease, to promote repetitive cycle DNA replication and exponentially increase the nucleic acid sequence. The amplification products form a Mg2+-DNAzyme unit with the specific primer, enabling real-time fluorescence detection of HIV-1 DNA through cyclic cleavage of hairpin probes. Under optimal conditions, this method has a detection limit of 10-17 M. It offers a simple design, isothermal conditions, and easy fluorescence measurement, making it highly sensitive and specific for quantitative detection of DNA or RNA in theoretical and clinical studies.

SENSORS AND ACTUATORS B-CHEMICAL (2023)

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Article Chemistry, Analytical

Development of a hybridization chain reaction-powered lab-on-fiber device for ultrafast point-of-care testing of circulating tuor DNA in whole blood

Jiaxin Xu et al.

Summary: Circulating tumor DNA (ctDNA) has shown promising potential in the prognosis, diagnosis, and surveillance of cancers, necessitating the need for rapid and sensitive point-of-care testing (POCT) technologies. Hybridization chain reaction (HCR)-based optical biosensors offer excellent solutions, but their scalability and clinical applications are hindered by the requirement of expensive optical readout devices, long detection time, and heating. This study presents an innovative HCR-powered lab-on-fiber device (HCR-LOFD) for rapid on-site detection of ctDNA with high sensitivity, specificity, and reproducibility. The HCR-LOFD combines HCR, fluorescence energy resonant transfer, and the evanescent wave fluorescence principle to achieve quantitative detection of KRAS G12D in 5 minutes at room temperature, simplifying optical readout devices and potentially serving as a solution for biomarker detection in limited medical resource regions.

TALANTA (2023)

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Article Biophysics

A portable system for isothermal amplification and detection of exosomal microRNAs

Jingjing Qian et al.

Summary: This paper presents an on-chip exosomal miRNA amplification and detection system for rapid analysis, which quantitatively measures miRNAs extracted from exosomes using the EXPAR assay. The system is designed to allow storage of multiple oligonucleotide templates for the EXPAR, sample and reagent mixing, and simultaneous analysis of multiple exosomal miRNAs.

BIOSENSORS & BIOELECTRONICS (2022)

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Review Chemistry, Analytical

Isothermal nucleic acid amplification for food safety analysis

Xuhan Xia et al.

Summary: This review outlines the application of isothermal amplification techniques in food safety analysis, including the detection of foodborne pathogens and authenticity analysis, the detection of heavy metals, mycotoxins, and antibiotics, and the imaging methods for in situ analysis of foodborne pathogens enabled by isothermal amplification.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2022)

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Review Chemistry, Analytical

Biosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring

Sandra Leonardo et al.

Summary: This article elaborates on the application of biosensors based on isothermal DNA amplification methods in the detection of bacteria in food safety and environmental monitoring. Various amplification techniques' advantages and limitations are described, indicating that while further efforts are needed, the combination of these techniques offers multiple possibilities for efficient bacteria detection.

SENSORS (2021)

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Article Chemistry, Analytical

Cost-Effective Multiplex Fluorescence Detection System for PCR Chip

Sung-Hun Yun et al.

Summary: This paper proposes a compact and cost-effective fluorescence detection system that can be integrated to a multiplex real-time PCR equipment. The system utilizes an open platform camera with embedded lens and a compact filter wheel, which performs comparably to photodiode systems in fluorescence detection.

SENSORS (2021)

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Article Biochemistry & Molecular Biology

Rapid and Sensitive Diagnosis of Drug-Resistant FLT3-F691L Mutation by CRISPR Detection

Yin Liu et al.

Summary: This study developed an accurate and sensitive FLT3-F691L diagnostic method by CRISPR detection, which can complete the whole diagnosis process within 40 minutes with excellent sensitivity, providing guidance for AML treatment.

FRONTIERS IN MOLECULAR BIOSCIENCES (2021)

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Letter Virology

CRISPR/Cas12a Technology Combined with RT-ERA for Rapid and Portable SARS-CoV-2 Detection

Sihua Liu et al.

VIROLOGICA SINICA (2021)

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Article Biochemical Research Methods

Real-time isothermal DNA amplification monitoring in picoliter volumes using an optical fiber sensor

Monika Janik et al.

Summary: This study demonstrates the potential of utilizing an optical fiber-fabricated unlabeled microcavity in-line Mach-Zehnder interferometer for real-time monitoring of DNA isothermal amplification, showing promising application in disease diagnosis.

LAB ON A CHIP (2021)

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Article Medicine, General & Internal