期刊
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
卷 18, 期 1, 页码 3-17出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1386207318666141205112730
关键词
Biochemical assays; cytokinesis; HTS; MgcRacGAP; Rac1; small molecule inhibitor
资金
- Jane and Aatos Erkko Foundation
- NIH [1 R03 DA033983]
- Helsinki Biomedical Graduate Program
- NATIONAL INSTITUTE ON DRUG ABUSE [R03DA033983] Funding Source: NIH RePORTER
The Rho family of Ras superfamily small GTPases regulates a broad range of biological processes such as migration, differentiation, cell growth and cell survival. Therefore, the availability of small molecule modulators as tool compounds could greatly enhance research on these proteins and their biological function. To this end, we designed a biochemical, high throughput screening assay with complementary follow-up assays to identify small molecule compounds inhibiting MgcRacGAP, a Rho family GTPase activating protein involved in cytokinesis and transcriptionally upregulated in many cancers. We first performed an in-house screen of 20,480 compounds, and later we tested the assay against 342,046 compounds from the NIH Molecular Libraries Small Molecule Repository. Primary screening hit rates were about 1% with the majority of those affecting the primary readout, an enzyme-coupled GDP detection assay. After orthogonal and counter screens, we identified two hits with high selectivity towards MgcRacGAP, compared with other RhoGAPs, and potencies in the low micromolar range. The most promising hit, termed MINC1, was then examined with cell-based testing where it was observed to induce an increased rate of cytokinetic failure and multinucleation in addition to other cell division defects, suggesting that it may act as an MgcRacGAP inhibitor also in cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据