期刊
SCIENTIA PHARMACEUTICA
卷 91, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/scipharm91030042
关键词
Eugenia uniflora; flavonol glycosides; myricitrin; cytotoxicity; antioxidant; anti-inflammatory
The leaves of Eugenia uniflora have been used in traditional medicine for the treatment of tumors and chronic inflammatory diseases. Two flavonol glycosides were isolated from the leaves, among which myricitrin showed potential cytotoxic and antioxidant activities, suggesting its potential as a cytotoxic agent.
In view of the extensive use of Eugenia uniflora leaves for the management of tumours and other chronic inflammatory diseases in traditional medicine, an activity-guided fractionation of its leaf ethanolic extract led to the isolation of two flavonol glycosides. Cytotoxicity study was based on the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) viability assay against the non-tumourigenic human embryonic kidney (HEK-293) cells, and the cancerous liver (Hep-G2) and cervical (HeLa) cell lines. Antioxidant tests were carried out using 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) and hydrogen peroxide (H2O2) radical scavenging assays, while an in vitro anti-inflammatory test was conducted using egg albumin denaturation (EAD) assay. Based on comprehensive spectroscopic and spectrometric evidence, the compounds were elucidated as myricitrin (1) and a newly described compound, 5,7-dihydroxy-3-(3,4,5-trihydroxy-6-methyltetrahydropyran-2-yloxy)-2-(2,4,5-trihydroxyphenyl)chromen-4-one, named unifloratrin (2). The cytotoxicity of myricitrin (1) was comparable to 5-fluorouracil (standard drug), with a CC50 of 8.5 +/- 2.2 mu g/100 mu L against HeLa cells. It also demonstrated better antioxidant activity, with an IC50 of 6.23 +/- 1.09, 22.01 +/- 2.59 and 30.46 +/- 1.79 mu M against DPPH, NO and H2O2 free radicals, respectively. At 20 mu g/mL and an incubation time of 2 h, myricitrin was comparable to diclofenac (standard drug) in anti-inflammatory activity. This report may serve as a justification for the ethnomedicinal use of E. uniflora, while flavonol glycosides, such as myricitrin (1), could be further exploited as a candidate cytotoxic agent.
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