Isolation of a newly Trichoderma asperellum LYS1 with abundant cellulase-hemicellulase enzyme cocktail for lignocellulosic biomass degradation

Article Biochemistry & Molecular Biology

Chitin Biodegradation by Lytic Polysaccharide Monooxygenases from Streptomyces coelicolor In Vitro and In Vivo

Fei Li et al.

Summary: This study found that the lpmo gene Sclpmo10G in Streptomyces coelicolor A3(2) strain is strongly induced by chitin, suggesting that ScLPMO10G may play a role in chitin biodegradation and have potential applications in biorefineries.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

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Article Agriculture, Dairy & Animal Science

Production, purification, characterization and application of two novel endoglucanases from buffalo rumen metagenome

Zhenxiang Meng et al.

Summary: This study characterized two endoglucanases, TrepCel3 and TrepCel4, and investigated the effect of their combination on in vitro rumen fermentation. The combination of TrepCel3 and TrepCel4 showed a synergistic relationship and significantly increased the saccharification yield of lignocellulosic substrates. It also enhanced gas, NH3-N, volatile fatty acid (VFA) production, and microbial protein synthesis during in vitro rumen fermentation. This combination could be a potential feed additive to improve agricultural residues utilization in ruminants.

JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY (2023)

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Article Agricultural Engineering

Synergetic effect of lytic polysaccharide monooxygenase from Thermobifida fusca on saccharification of agrowastes

Hyun June Park et al.

Summary: Saccharification is an important process in biomass-based biorefineries. This study focuses on optimizing the expression level of a bacterial lytic polysaccharide monooxygenase from Thermobifida fusca (TfLPMO), which is a cellulolytic enzyme, and investigating its synergistic effect with a commercial cellulase cocktail on the saccharification of agrowaste. The results show that the combination of TfLPMO with cellulase increases the reducing sugars from rice straw and corncob, suggesting the potential of valorizing agrowastes as renewable feedstocks in biorefineries.

BIORESOURCE TECHNOLOGY (2023)

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Article Agricultural Engineering

BsEXLX of engineered Trichoderma reesei strain as dual-active expansin to boost cellulases secretion for synergistic enhancement of biomass enzymatic saccharification in corn and Miscanthus straws

Chengbao Xu et al.

Summary: In this study, the BsEXLE1 gene was overexpressed in T. reesei to create the TrEXLX10 strain, which showed increased activities of beta-glucosidases, cellobiohydrolases, and xylanses compared to the Rut-C30 strain. The enzymes secreted by TrEXLX10 resulted in higher yields of hexoses in biomass saccharification experiments. Furthermore, the study identified the potential of expansin, purified from the TrEXLX10 secreted solution, to enhance cellulose hydrolysis independently.

BIORESOURCE TECHNOLOGY (2023)

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Article Biotechnology & Applied Microbiology

Assessing rhizosphere Trichoderma asperellum strains for root colonizing and antagonistic competencies against Fusarium wilt through molecular and biochemical responses in castor

Doris A. Pradhan et al.

Summary: The study aimed to identify effective strains of Trichoderma asperellum for colonizing castor roots, promoting growth, and limiting pathogen infection. The strains 7316 and N13 were found to have strong antagonistic competencies against Fusarium oxysporum through dual culture-confrontation and in-planta assays, and they also showed high mycoparasitic propensity. Biochemical analysis revealed that Trichoderma can protect against oxidative stress damage. Therefore, these strains could be utilized as potential biocontrol agents to enhance growth and confer resistance to Fusarium oxysporum ricini.

BIOLOGICAL CONTROL (2023)

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Article Agricultural Engineering

Achieving high enzymatic hydrolysis sugar yield of sodium hydroxide-pretreated wheat straw with a low cellulase dosage by adding sulfomethylated tannic acid

Shihan Hu et al.

Summary: Sulfonated lignin enhances enzymatic hydrolysis of lignocellulose substrates. Sulfonated polyphenols, such as tannic acid, may have similar effects. Sulfomethylated tannic acids (STAs) with different sulfonation degrees were prepared and investigated for their impact on enzymatic saccharification. STAs significantly promoted the substrate enzymatic digestibility, providing a reliable approach for establishing an efficient lignocellulosic enzyme hydrolysis system.

BIORESOURCE TECHNOLOGY (2023)

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Article Chemistry, Multidisciplinary

Compartmentalization of a synergistic fungal-bacterial consortium to boost lactic acid conversion from lignocellulose via consolidated bioprocessing

Yujia Jiang et al.

Summary: This study successfully increased the efficiency of cellulose acid production from 7.36 g L-1 (native microbial consortium) to 57.57 g L-1 by re-assembling a simplified microbial consortium consisting of lignocellulose degrader Trichoderma asperellum and lactic acid (LA) producer Lactobacillus paracasei. The analysis of interactions between these two members provides insights for the construction of synthetic microbial consortia for the synthesis of other chemicals.

GREEN CHEMISTRY (2023)

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Article Agricultural Engineering

Comparison of sulfomethylated lignin from poplar and masson pine on cellulase adsorption and the enzymatic hydrolysis of wheat straw

Mohan Li et al.

Summary: The addition of sulfomethylated lignins in enzymatic hydrolysis significantly improved substrate digestibility and sugar yield. Competitive adsorption between cellulase, substrate lignin, and SLs was suggested as a mechanism for the observed effects.

BIORESOURCE TECHNOLOGY (2022)

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Editorial Material Biotechnology & Applied Microbiology

Thermophiles: potential chassis for lignocellulosic biorefinery

Yujia Jiang et al.

Summary: Lignocellulosic thermophiles can accelerate lignocellulose degradation, and constructing synthetic microbial consortia is a potential strategy to improve the efficiency of lignocellulosic biorefineries.

TRENDS IN BIOTECHNOLOGY (2022)

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Article Environmental Sciences

Enhanced Cellulase Production by Talaromyces amestolkiae CMIAT055 Using Banana Pseudostem

Genilton S. Faheina Junior et al.

Summary: This study aimed to enhance the production of cellulases by Talaromyces amestolkiae CMIAT 055 through the optimization of culture medium components and agitation configurations in a bioreactor. Statistical experimental design and studies of pH control and agitation system were conducted. The results showed that optimizing the components of the culture medium and using a Pitched blade impeller at 300 rpm with pH maintained at 5.0 resulted in a significant increase in cellulase activity. The use of lignocellulosic materials as inducers also proved to be a cost-effective strategy for enzyme production.

WASTE AND BIOMASS VALORIZATION (2022)

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Article Microbiology

Ultrahigh-Throughput Screening of High-β-Xylosidase-Producing Penicillium piceum and Investigation of the Novel β-Xylosidase Characteristics

Zhaokun Zhang et al.

Summary: A droplet-based microfluidic ultrahigh-throughput screening technology was developed to select high-beta-xylosidase-producing Penicillium piceum W6 from a mutated library. A novel beta-D-xylosidase with bifunctional activities was purified and identified from this strain. The addition of this enzyme significantly enhanced the yields of glucose and xylose from delignified biomass.

JOURNAL OF FUNGI (2022)

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Article Biotechnology & Applied Microbiology

Xylanase Production by Talaromyces amestolkiae Valuing Agroindustrial Byproducts

Giorgia S. Barbieri et al.

Summary: This study evaluated the production of xylanase by the filamentous fungi Talaromyces amestolkiae using different agroindustrial byproducts. The results showed that wheat bran, which contains a high amount of xylan, generated the highest enzymatic activity. By optimizing the culture conditions, such as adding yeast extract and K2HPO4, the xylanase production increased by 141%.

BIOTECH (2022)

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Article Agricultural Engineering

Alleviating product inhibition of Trichoderma reesei cellulase complex with a product-activated mushroom endoglucanase

Gen Zou et al.

Summary: Introducing product-activated cellulase genes significantly increases enzyme activity and saccharification efficiency. Improving thermostability of cellulases was achieved through mutants and enhanced enzyme activity. Introducing product-activated cellulase genes is an effective approach to alleviate product inhibition of cellulases.

BIORESOURCE TECHNOLOGY (2021)

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Article Microbiology

Biocontrol and growth-promoting effect of Trichoderma asperellum TaspHu1 isolate from Juglans mandshurica rhizosphere soil

Zeyang Yu et al.

Summary: The study focused on collecting, identifying, and evaluating native Trichoderma strains, leading to the discovery of TaspHu1 which demonstrated excellent adaptability and biocontrol abilities for promoting growth and health of tomato seedlings. Therefore, it is a promising biocontrol candidate for agricultural and forestry applications.

MICROBIOLOGICAL RESEARCH (2021)

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Review Biotechnology & Applied Microbiology

An overview of Trichoderma reesei co-cultures for the production of lignocellulolytic enzymes

Guilherme Bento Sperandio et al.

Summary: Biorefineries are essential for a sustainable circular bioeconomy, utilizing microbial enzymes to hydrolyze lignocellulosic substrates. Fungal co-cultures have shown enhanced enzyme activities, especially in beta-glucosidase, with Trichoderma reesei being a prominent candidate for future investigations. Further research is needed to explore molecular interactions and optimize enzyme cocktails in controlled environments before industrial application.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2021)

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Article Biotechnology & Applied Microbiology

Rational engineering of xylanase hyper-producing system in Trichoderma reesei for efficient biomass degradation

Su Yan et al.

Summary: In this study, a xylanase hyper-producing system in T. reesei was established by manipulating transcription factors and overexpressing xylanase, leading to efficient xylan hydrolysis and improved biomass degradation efficiency.

BIOTECHNOLOGY FOR BIOFUELS (2021)

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Article Biotechnology & Applied Microbiology

Synergistic mechanism of GH11 xylanases with different action modes from Aspergillus niger An76

Shu Zhang et al.

Summary: Aspergillus niger An76 encodes a comprehensive set of xylan-degrading enzymes, including five endo-xylanases (one GH10 and four GH11). Quantitative transcriptional analysis showed that three xylanase genes (xynA, xynB, and xynC) were up-regulated by xylan substrates, with different secretion orders and amounts. Among the GH11 xylanases, XynB demonstrated the best performance in xylan degradation, and synergistic effects were observed between XynA and XynB.

BIOTECHNOLOGY FOR BIOFUELS (2021)

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Article Biochemistry & Molecular Biology

Identification patterns of Trichoderma strains using morphological characteristics, phylogenetic analyses and lignocellulolytic activities

Azriah Asis et al.

Summary: Trichoderma is a soil-borne fungus genus with economic importance in agriculture, and its accurate identification is crucial for commercial purposes. Using a three-pronged approach based on morphological characteristics and multilocus gene sequences, 53 Trichoderma strains from Malaysia were identified as belonging to three different species. The phylogenetic analysis revealed distinct groups within the species, and measuring lignocellulolytic activities proved to be a valuable diagnostic tool for identifying Trichoderma strains for potential agricultural applications.

MOLECULAR BIOLOGY REPORTS (2021)

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Article Agricultural Engineering

Addressing challenges in production of cellulases for biomass hydrolysis: Targeted interventions into the genetics of cellulase producing fungi

Rajeev K. Sukumaran et al.

Summary: Fungal biomass degrading enzymes are crucial for biorefineries, but limited by the cost of biomass hydrolyzing enzymes. Current research focuses on improving the quantity and quality of enzymes secreted by fungi. Further production enhancements require modifying fungal metabolism.

BIORESOURCE TECHNOLOGY (2021)

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Article Biotechnology & Applied Microbiology

An engineered cellobiohydrolase I for sustainable degradation of lignocellulosic biomass

Subrata Pramanik et al.

Summary: This study successfully engineered the cellobiohydrolase I (CBH-I) from Penicillium verruculosum to improve thermostability and tolerance in various solvents without compromising its activity. The engineered triple variant CBH-I R1 demonstrated increased specific activities in different solvents and higher hydrolytic efficiency for lignocellulosic biomass degradation.

BIOTECHNOLOGY AND BIOENGINEERING (2021)

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Article Biotechnology & Applied Microbiology

Microbial enrichment and meta-omics analysis identify CAZymes from mangrove sediments with unique properties

Douglas Antonio Alvaredo Paixao et al.

Summary: The study used an enrichment process and integrative omics approaches to identify new lignocellulose-degrading enzymes, revealing that the lignocellulolytic-enriched mangrove microbial community can efficiently degrade sugarcane bagasse and harbor a large number of CAZyme sequences, including cellulases and hemicellulases.

ENZYME AND MICROBIAL TECHNOLOGY (2021)

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Article Biotechnology & Applied Microbiology

Structural insights into xylanase mutant 254RL1 for improved activity and lower pH optimum

La Xiang et al.

Summary: The engineered xylanase showed improved activity at a lower pH, with structural analysis revealing mutations that enlarged and shortened the access tunnel, changed the hydrogen bond network and decreased the pK(a) value, leading to a decreased pH optimum. This provides a basis for acid-alkaline engineering of glycoside hydrolases.

ENZYME AND MICROBIAL TECHNOLOGY (2021)

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Article Genetics & Heredity

Draft genome of the glucose tolerant β-glucosidase producing rare Aspergillus unguis reveals complete cellulolytic machinery with multiple beta-glucosidase genes

Prajeesh Kooloth-Valappil et al.

Summary: The draft genome sequence of the rare fungus Aspergillus unguis NII 08,123, producing glucose-tolerant beta glucosidase (GT-BGL), was generated using Next Generation Sequencing (NGS). The genome size was estimated to be 37.1 Mb, with 15,161 predicted proteins. Analysis of CAZyme genes revealed differences in lignocellulose degrading enzyme distribution between A. unguis and Trichoderma reesei, potentially due to tight regulation of cellulolytic machinery in the former. The fungus contained 403 predicted CAZymes, including various classes such as glycoside hydrolases, carbohydrate esterases, glycosyl transferases, polysaccharide lyases, and genes with auxiliary activities.

FUNGAL GENETICS AND BIOLOGY (2021)

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Article Agricultural Engineering

Promoting cellulase and hemicellulase production from Trichoderma orientalis EU7-22 by overexpression of transcription factors Xyr1 and Ace3

Yong Xue et al.

BIORESOURCE TECHNOLOGY (2020)

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Review Biotechnology & Applied Microbiology

Designing a cellulolytic enzyme cocktail for the efficient and economical conversion of lignocellulosic biomass to biofuels

Mukund Adsul et al.

ENZYME AND MICROBIAL TECHNOLOGY (2020)

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Article Biochemistry & Molecular Biology

Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

Yu Zhang et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

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Article Biotechnology & Applied Microbiology

The contribution of specific subsites to catalytic activities in active site architecture of a GH11 xylanase

Xiuyun Wu et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2020)

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Article Biochemistry & Molecular Biology

Enhanced production of cellulase from a novel strain Trichoderma gamsii M501 through response surface methodology and its application in biomass saccharification

Ramiya Baskaran et al.

PROCESS BIOCHEMISTRY (2020)

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Review Biotechnology & Applied Microbiology

Cellulosic ethanol production: Progress, challenges and strategies for solutions

Chen-Guang Liu et al.

BIOTECHNOLOGY ADVANCES (2019)

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Review Biochemistry & Molecular Biology

Microbial Beta Glucosidase Enzymes: Recent Advances in Biomass Conversation for Biofuels Application

Neha Srivastava et al.

BIOMOLECULES (2019)

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Article Agricultural Engineering

Improvement of cellulase production in Trichoderma reesei Rut-C30 by overexpression of a novel regulatory gene Trvib-1

Fei Zhang et al.

BIORESOURCE TECHNOLOGY (2018)

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Article Agricultural Engineering

Sequential and simultaneous strategies for biorefining of wheat straw using room temperature ionic liquids, xylanases and cellulases

Eric Husson et al.

BIORESOURCE TECHNOLOGY (2018)

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Article Biochemistry & Molecular Biology

Gene cloning, characterization and thermodynamic analysis of a novel multidomain hyperthermophilic GH family 3 β-glucosidase (TnBglB) from Thermotoga naphthophila RKU-10T

Fatima Akram et al.

PROCESS BIOCHEMISTRY (2018)

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Article Biotechnology & Applied Microbiology

Genomics insights into different cellobiose hydrolysis activities in two Trichoderma hamatum strains

Peng Cheng et al.

MICROBIAL CELL FACTORIES (2017)

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Article Biotechnology & Applied Microbiology

Carbohydrate-active enzymes in Trichoderma harzianum: a bioinformatic analysis bioprospecting for key enzymes for the biofuels industry

Jaire Alves Ferreira Filho et al.

BMC GENOMICS (2017)

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Article Biotechnology & Applied Microbiology

Differential β-glucosidase expression as a function of carbon source availability in Talaromyces amestolkiae: a genomic and proteomic approach

Laura I. de Eugenio et al.

BIOTECHNOLOGY FOR BIOFUELS (2017)

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Article Biotechnology & Applied Microbiology

Recombinant expression of thermostable processive MtEG5 endoglucanase and its synergism with MtLPMO from Myceliophthora thermophila during the hydrolysis of lignocellulosic substrates

Anthi Karnaouri et al.

BIOTECHNOLOGY FOR BIOFUELS (2017)

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Review Biotechnology & Applied Microbiology

Insights into the mechanism of enzymatic hydrolysis of xylan

L. R. S. Moreira et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2016)

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Article Biotechnology & Applied Microbiology

A β-glucosidase hyper-production Trichoderma reesei mutant reveals a potential role of cel3D in cellulase production

Chengcheng Li et al.

MICROBIAL CELL FACTORIES (2016)

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Article Agricultural Engineering

Isolation and characterization of a novel Penicillium oxalicum strain Z1-3 with enhanced cellobiohydrolase production using cellulase-hydrolyzed sugarcane bagasse as carbon source

Lei Jing et al.

INDUSTRIAL CROPS AND PRODUCTS (2015)

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Article Biotechnology & Applied Microbiology

Isolation and Characterization of a Novel Thermostable β-glucosidase from Bacillus subtilis SU40

B. M. Asha et al.

APPLIED BIOCHEMISTRY AND MICROBIOLOGY (2015)

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Article Biochemistry & Molecular Biology

Enhanced Xylanase Performance in the Hydrolysis of Lignocellulosic Materials by Surfactants and Non-catalytic Protein

Xiaoyan Ge et al.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2014)

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Article Biochemistry & Molecular Biology

The carbohydrate-active enzymes database (CAZy) in 2013

Vincent Lombard et al.

NUCLEIC ACIDS RESEARCH (2014)

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Article Biochemistry & Molecular Biology

Crystal structures of glycoside hydrolase family 3 β-glucosidase 1 from Aspergillus aculeatus

Kentaro Suzuki et al.

BIOCHEMICAL JOURNAL (2013)

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Article Agricultural Engineering

A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology

Tomohisa Hasunuma et al.

BIORESOURCE TECHNOLOGY (2013)

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Review Agricultural Engineering

Role and significance of beta-glucosidases in the hydrolysis of cellulose for bioethanol production

Reeta Rani Singhania et al.

BIORESOURCE TECHNOLOGY (2013)

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Review Biotechnology & Applied Microbiology

Production of recombinant proteins by filamentous fungi

Owen P. Ward

BIOTECHNOLOGY ADVANCES (2012)

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Review Biotechnology & Applied Microbiology

Carbohydrate-binding domains: multiplicity of biological roles

Daniel Guillen et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2010)

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Review Biotechnology & Applied Microbiology

Trends and challenges in the microbial production of lignocellulosic bioalcohol fuels

Christian Weber et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2010)

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Isolation of a newly Trichoderma asperellum LYS1 with abundant cellulase-hemicellulase enzyme cocktail for lignocellulosic biomass degradation

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