Self-assembled condensed tannins supramolecular system can adsorb cholesterol micelles to promote cholesterol excretion

In this study, the cholesterol (CH)-lowering behavioral mechanisms and drivers of condensed tannins (CTs) were revealed using a molecular aggregation theoretical model combined with in vitro experiments, as well as the CH-lowering effects of CTs validated based on animal experiments. Theoretical model results indicated that CTs can spontaneously aggregate to form supramolecular systems, can break CH micelles and form larger aggregates, a behavior driven by van der Waals forces and hydrogen bonds; DLS and TEM results confirmed that the presence of CH leads to a larger particle size of CTs and the formation of large aggregates; thermodynamic analysis and ITC revealed that the adsorption of CH by CTs is a spontaneous reaction driven by hydrogen bonds and hy-drophobic forces; Animal experiments and fecal biochemical parameters further confirmed that the intake of CTs can reduce CH absorption and promotes CH excretion. Overall, this study reveals the CH-lowering behavioral mechanism of CTs from the perspective of molecular aggregation behavior.

Automated summary

This study reveals the behavioral mechanisms and drivers of condensed tannins (CTs) in lowering cholesterol, using a molecular aggregation theoretical model combined with in vitro and animal experiments. It is shown that CTs can aggregate to break cholesterol micelles and form larger aggregates, driven by van der Waals forces and hydrogen bonds. The intake of CTs is found to reduce cholesterol absorption and promote cholesterol excretion.