Optimization and purification of bioproducts from Bacillus velezensis PhCL fermentation and their potential on industrial application and bioremediation

Bioproduction is considered a promising alternative way of obtaining useful and green chemicals. However, the downstream process of biomolecules has been one of the major difficulties in upscaling the application of bioproducts due to the high purification cost. Acid precipitation is the most common method for purifying biosurfactants from the fermentation broth with high purity. However, the use of strong acids and organic solvents in solvent extraction has limited its application. Hence, in this study, a new strain of Bacillus velezensis PhCL was isolated from phenolic waste, and its production of amylase had been optimized via response surface methodology. After that, amylase and biosurfactant were purified by sequential ammonium sulfate precipitation and the result suggested that even though the purified crude biosurfactant had a lower purification fold compared to the acid precipitation, the yield was higher and both enzymes and biosurfactant also could be recovered for lowering the purification cost. Moreover, the purified amylase and crude biosurfactant were characterized and the results suggested that the purified crude biosurfactant would have a higher emulsion activity and petroleum hydrocarbon removal rate compared to traditional surfactants. This study provided another approach for purifying bioactive compounds including enzymes and biosurfactants from the same fermentation broth and further explored the potential of the crude purified biosurfactant in the bioremediation of polycyclic aromatic hydrocarbons and petroleum hydrocarbons.

Automated summary

Bioproduction is a promising approach to obtain useful and green chemicals. However, the high purification cost of biomolecules in the downstream process has been a major challenge for scaling up the application of bioproducts. In this study, a new strain of Bacillus velezensis was isolated and optimized for the production of amylase. The enzymes and biosurfactant were then purified using sequential ammonium sulfate precipitation, which showed a higher yield compared to traditional methods. The purified biosurfactant exhibited higher emulsion activity and petroleum hydrocarbon removal rate than conventional surfactants. This study provides a new approach for purifying bioactive compounds and explores the potential of purified biosurfactant in bioremediation.