期刊
POSTHARVEST BIOLOGY AND TECHNOLOGY
卷 206, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.postharvbio.2023.112547
关键词
Ascorbic acid; Broccoli; Glucosinolate; Glutathione; Multi-omics; Reactive oxygen species
This study investigated the effects of exogenous H2O2 on antioxidant metabolism and yellowing in postharvest broccoli. The results showed that exogenous H2O2 promoted the accumulation of endogenous H2O2 and up-regulated the expressions of antioxidant genes, leading to enhanced scavenging of reactive oxygen species and alleviated oxidative damage. Additionally, H2O2 treatment also promoted the biosynthesis of antioxidant compounds and enhanced the health-care function of postharvest broccoli.
To investigate the effects and potential mechanism of exogenous H2O2 on antioxidant metabolism and yellowing in postharvest broccoli, the transcriptome and metabolome integrated analysis were performed. A total of 61 differentially expressed genes and 6 differentially accumulated metabolites identified among of H2O2 and diphenylene iodonium (DPI) treatments on 2 and 4 d. Exogenous H2O2 promoted the accumulation of endogenous H2O2 within 1 d by activating RBOHs expressions.H2O2 treatment up-regulated the expressions of SOD, CAT and peroxiredoxin (Prx) genes and APX, DHAR and GPX genes involved in ascorbic acid-glutathione (AsA-GSH) cycle, thereby promoting the scavenging of O-2 '(-) and H2O2 and alleviating the oxidative damage. Additionally, H2O2 treatment promoted the biosynthesis of AsA and GSH, and increased the content of AsA, GSH, oxidized glutathione, and.-glutamylcysteine.H2O2 treatment also promoted glucosinolate biosynthesis, the accumulation of 3-(6'-methylthio)hexylmalic acid, and the production of sulforaphane by up-regulating MYS expression, thereby enhancing the health-care function of postharvest broccoli. This study provided the new insights into regulatory mechanism of H2O2 on antioxidant system.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据