Fluorescent polythymidine-templated copper nanoclusters aptasensor for sensitive detection of tropomyosin in processed shrimp products

Article Food Science & Technology

A ratiometric fluorescent aptasensor based on EXPAR to detect shellfish tropomyosin in food system

Jinru Zhou et al.

Summary: A novel switch-conversional ratiometric fluorescent biosensor was developed to accurately detect shellfish allergens in complex food systems, providing technical support for allergen label supervision.

FOOD CONTROL (2023)

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Article Chemistry, Applied

Nanocellulose-based polymeric nanozyme as bioinspired spray coating for fruit preservation

Lunjie Huang et al.

Summary: This study presents a biocatalytic spraying coating for fruit preservation, using banana and mango as model fruits. The biohybrid nanofibers in the coating showed excellent antibacterial performance and effectively killed foodborne pathogens, thereby improving the quality of the fruits.

FOOD HYDROCOLLOIDS (2023)

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Article Spectroscopy

Discrimination of Pericarpium Citri Reticulatae in different years using Terahertz Time-Domain spectroscopy combined with convolutional neural network

Yao Liu et al.

Summary: This study investigated the feasibility of using terahertz time-domain spectroscopy combined with a convolutional neural network to identify different year Pericarpium Citri Reticulatae samples. The results showed that the CNN model had better performance than other models, and it could rapidly and accurately identify the quality of Pericarpium Citri Reticulatae.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2023)

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Article Spectroscopy

Mesoporous silica coated core-shell nanoparticles substrate for size-selective SERS detection of chloramphenicol

Heera Jayan et al.

Summary: In this study, a novel plasmonic nanoparticles substrate with a mesoporous coating was synthesized for selective enhancement and detection of small molecules. The substrate showed high sensitivity and reproducibility, achieving the detection of chloramphenicol in milk samples. The addition of the mesoporous coating provided additional stability to the nanoparticles, allowing for substrate reusability.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2023)

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Article Chemistry, Analytical

A dual signal-on biosensor based on dual-gated locked mesoporous silica nanoparticles for the detection of Aflatoxin B1

Zhihui Wu et al.

Summary: In this study, a dual signal-on biosensor based on surface-enhanced Raman scattering (SERS) and fluorescence was developed for the detection of highly toxic mycotoxin Aflatoxin B1 (AFB1). The biosensor exhibited high sensitivity and specificity, and can be applied for the detection of AFB1 in real food samples.

TALANTA (2023)

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Article Chemistry, Analytical

A SERS-Fluorescence dual-signal aptasensor for sensitive and robust determination of AFB1 in nut samples based on Apt-Cy5 and MNP@Ag-PEI

Haoyang He et al.

Summary: In this study, a dual-signal aptasensor combining surface-enhanced Raman scattering and fluorescence signals was developed for sensitive detection of AFB1 contamination in nut samples. The aptasensor showed satisfactory linear ranges and detection limits for both SERS and fluorescence methods, and achieved high recovery rates in the nut samples.

TALANTA (2023)

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Article Chemistry, Analytical

A novel fluorescence biosensor based on CRISPR/Cas12a integrated MXenes for detecting Aflatoxin B1

Zhihui Wu et al.

Summary: A novel fluorescence biosensor based on CRISPR/Cas12a and MXenes was developed for AFB1 detection in food. The biosensor showed a wide detection range, low detection limit, and rapid detection time, and demonstrated excellent detection performance in real samples.

TALANTA (2023)

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Review Food Science & Technology

Precision release systems of food bioactive compounds based on metal-organic frameworks: synthesis, mechanisms and recent applications

Mingchun Lv et al.

Summary: Controlled release systems using metal-organic frameworks (MOFs) as carriers have shown great potential in effectively delivering bioactive compounds (BCs) with improved solubility, stability, and biocompatibility, expanding their applications in the food industry. Encapsulation of BCs by MOFs and control of their release have been successfully achieved, contributing to maintaining or enhancing the quality and safety of food while reducing BCs usage and increasing bioavailability. Low- or non-biotoxic MOFs, especially bio-MOFs, hold promising prospects for applications in the food industry.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2022)

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Article Spectroscopy

Development of core-satellite-shell structured MNP@Au@MIL-100(Fe) substrates for surface-enhanced Raman spectroscopy and their applications in trace level determination of malachite green in prawn

Hongbin Pu et al.

Summary: In this study, SERS active MNP@Au@MIL-100(Fe) substrates were developed to monitor malachite green residues in aquatic products. The use of metal-organic frameworks (MOFs) shell as a filter effectively avoided food matrix interference and improved the identification ability to analytes.

JOURNAL OF RAMAN SPECTROSCOPY (2022)

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Article Food Science & Technology

On-off-on fluorescent nanosensing: Materials, detection strategies and recent food applications

Haoyang He et al.

Summary: On-off-on fluorescent sensors based on nanomaterials have gained much attention in food analysis due to their high sensitivity, wide detection range, strong anti-interference ability, and flexible design. This review introduces common nanomaterials and elaborates the main detection strategies of on-off-on fluorescent sensors. It also summarizes recent applications of these sensors for trace level detection of various analytes in food. However, the commercial and industrial applications of these sensors are still limited, and future research should focus on developing high-throughput detection techniques and portable or visual fluorescent sensors.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2022)

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Review Food Science & Technology

Recent developments in Raman spectral analysis of microbial single cells: Techniques and applications

Heera Jayan et al.

Summary: Single-cell analysis reveals the functional and structural variations of each cell, eliminating the need for traditional microbial cultivation techniques. Raman spectroscopy as a label-free, noninvasive method is ideal for spatially resolved chemical measurements at the single-cell level, offering important insights for microbial studies.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2022)

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Article Instruments & Instrumentation

Detection of Bioactive Metabolite in Escherichia Coli Culture Using Surface-Enhanced Raman Spectroscopy

Heera Jayan et al.

Summary: A SERS-based technique was developed for the detection of bioactive metabolite indole produced by E. coli. The use of highly sensitive nanoparticles allowed for detection of low concentrations of indole in biological media. Supplementation of growth media with exogenous tryptophan affected the production of indole and the growth of bacterial cells.

APPLIED SPECTROSCOPY (2022)

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Article Food Science & Technology

A fluorometric sandwich biosensor based on rationally imprinted magnetic particles and aptamer modified carbon dots for the detection of tropomyosin in seafood products

Yanbo Wang et al.

Summary: A sandwich biosensor using magnetic particles and carbon dots was proposed for the detection of TM in seafood products, with a detection limit of 30.76 ng mL(-1). The method demonstrated excellent specificity and accuracy in detecting TM in commercial samples, showing great potential for applications.

FOOD CONTROL (2022)

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Review Chemistry, Inorganic & Nuclear

DNA-templated coinage metal nanostructures and their applications in bioanalysis and biomedicine

Shenshan Zhan et al.

Summary: This review presents the synthesis of DNA-templated coinage metal nanostructures (CMNs) and their applications in bioanalysis and biomedicine. The field shows great potential in ion detection, nucleic acid detection, enzyme detection, biomolecule detection, and bioimaging.

COORDINATION CHEMISTRY REVIEWS (2022)

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Article Chemistry, Applied

Ti3C2Tx MXenes loaded with Au nanoparticle dimers as a surface-enhanced Raman scattering aptasensor for AFB1 detection

Zhihui Wu et al.

Summary: A ratiometric surface-enhanced Raman scattering (SERS) aptasensor for AFB1 detection was developed utilizing BPE to trigger the assembly of AuNP dimers and the interaction between MXenes nanosheets and aptamer-modified AuNP dimers. The sensor showed promising results in detecting AFB1 with a limit of detection (LOD) of 0.6 pg.mL(-1) in standard solution.

FOOD CHEMISTRY (2022)

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Article Chemistry, Applied

A competitive colorimetric aptasensor for simple and sensitive detection of kanamycin based on terminal deoxynucleotidyl transferase-mediated signal amplification strategy

Tingting Zhao et al.

Summary: We developed a rapid and sensitive colorimetric biosensor based on competitive recognition and signal amplification strategy, which can achieve high selective detection of kanamycin through competitive recognition with kanamycin and signal amplification.

FOOD CHEMISTRY (2022)

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Article Chemistry, Multidisciplinary

Photosensitized Peroxidase Mimicry at the Hierarchical 0D/2D Heterojunction-Like Quasi Metal-Organic Framework Interface for Boosting Biocatalytic Disinfection

Lunjie Huang et al.

Summary: This study demonstrates the design of a nanozyme Q-MOFCe0.5 through a hierarchical quasi-MOF scheme, which has the ability to generate sustained reactive oxygen species, effectively killing bacteria and disinfecting wounds.

SMALL (2022)

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Article Spectroscopy

Glutathione-stabilized copper nanoclusters mediated-inner filter effect for sensitive and selective determination of p-nitrophenol and alkaline phosphatase activity

Hai-Bo Wang et al.

Summary: A simple and highly selective fluorescence biosensor based on glutathione-stabilized copper nanoclusters (GSH-CuNCs) has been developed for the detection of p-nitrophenol (p-NP) and alkaline phosphatase (ALP) activity. The method shows promising sensitivity and broad application prospects.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2022)

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Article Chemistry, Multidisciplinary

Food Allergies: Immunosensors and Management

Dyah Ika Krisnawati et al.

Summary: This article discusses the mechanism, importance, and common allergenic foods of food allergies. It provides important information for children with allergic reactions to help them avoid allergenic foods.

APPLIED SCIENCES-BASEL (2022)

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Article Biochemistry & Molecular Biology

Aptamer Molecular Beacon Sensor for Rapid and Sensitive Detection of Ochratoxin A

Hao Yu et al.

Summary: A simple molecular aptamer beacon (MAB) sensor for rapid detection of the carcinogenic fungal secondary metabolite OTA has been developed. The sensor showed high selectivity and sensitivity, enabling detection of OTA in a wide dynamic concentration range. The method demonstrated capability for OTA analysis in practical applications.

MOLECULES (2022)

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Article Chemistry, Analytical

A volatile basic nitrogens-responsive tag based on aggregation-induced emission luminogen for real-time monitoring and in situ visualization of salmon freshness

Wenyang Zhang et al.

Summary: Given the increasing food safety issues, the establishment of efficient methods for monitoring food freshness has gained significant attention. In this study, a novel fluorescent probe using glutathione capped copper nanoclusters (GSH-CuNCs) aggregates was developed for real-time and visual monitoring of salmon freshness. The sensitivity and sensing mechanism of the GSH-CuNCs aggregates were evaluated, and a gas indicator based on the aggregates was fabricated for detecting ammonia vapor concentrations. The feasibility and practical applications of the GSH-CuNCs aggregates-based tag in the food industry were demonstrated.

ANALYTICA CHIMICA ACTA (2022)

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Article Chemistry, Applied

Visualization and quantification of content and hydrogen bonding state of water in apple and potato cells by confocal Raman microscopy: A comparison study

Dongmei Li et al.

Summary: This study developed an improved method to directly visualize the spatial distribution of water content and hydrogen bonding state in apple and potato cells, comparing the differences in water distribution. The results demonstrated that the method could successfully quantify the content and hydrogen bonding state of water in fruit and vegetable cells.

FOOD CHEMISTRY (2022)

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Article Chemistry, Multidisciplinary

High Fructose Concentration Increases the Fluorescence Stability of DNA-Templated Copper Nanoclusters by Several Thousand Times

Seokjoon Kim et al.

Summary: In this study, DNA-templated copper nanoclusters with improved fluorescence stability were prepared by introducing fructose into the synthesis process. The inclusion of fructose significantly increased the operating lifetime of the CuNCs and improved their stability against various conditions. Moreover, the fluorescence signal of CuNCs was maintained for a longer time when stored at low temperatures. These findings provide a basis for improving the stability of copper nanoclusters for various applications.

NANO LETTERS (2022)

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Article Spectroscopy

Analyzing macromolecular composition of E. Coli O157:H7 using Raman-stable isotope probing

Heera Jayan et al.

Summary: In this study, Raman spectroscopy combined with deuterium labelling was used to analyze the metabolic activity of Escherichia coli cells. The method could semi-quantitatively determine total metabolic activity, macromolecule specific identification, and lipid and protein metabolism in a single cell.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2022)

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Article Spectroscopy

Surface-enhanced Raman spectroscopy combined with stable isotope probing to assess the metabolic activity of Escherichia coli cells in chicken carcass wash water

Heera Jayan et al.

Summary: This study used surface-enhanced Raman spectroscopy combined with isotope probing to analyze the disinfection of single bacterial cells in chicken carcass wash water, showing that the presence of disinfectants significantly reduced the metabolic activity of bacteria. The research proved the potential of the proposed method in assessing microbial metabolic activity in other food products and optimizing the disinfection process.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2022)

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Review Food Science & Technology

Raman spectroscopic techniques for detecting structure and quality of frozen foods: principles and applications

Wenyang Zhang et al.

Summary: Raman spectroscopic techniques can assess the compositional, physicochemical, and sensory characteristics of frozen foods without any pretreatment, as well as investigate the structural alterations of protein, lipid, water and other components. The future development of Raman spectroscopy in monitoring frozen foods should focus on reducing overlapping signals, achieving real noninvasive detection, eliminating misclassification and quantification errors, establishing a standardized Raman database, and controlling cost.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2021)

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Article Biochemical Research Methods

Smartphone-assisted fluorescent analysis of polyT-Cu-nanoprobes using nucleic acid amplification test for the diagnosis of tuberculosis

Chung-An Chen et al.

Summary: This study demonstrated a new detection platform for tuberculosis diagnosis using smartphone-assisted fluorescent analysis and highly sensitive fluorescent copper nanoprobes. The platform showed high precision and accuracy in clinical samples, suggesting potential for practical application.

ANALYTICAL BIOCHEMISTRY (2021)

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Article Chemistry, Physical

Quantification of hydrogen bonding strength of water in saccharide aqueous solutions by confocal Raman microscopy

Dongmei Li et al.

Summary: In this study, Raman spectroscopy was used to investigate the quantitative relationship between the hydrogen bonding strength of water and saccharide aqueous solutions. The results showed that the enhancement effect of saccharide molecules on the hydrogen bonding strength of water increased with concentration. The proposed equation based on the average number of hydrogen bonds of water molecule provided a satisfactory accuracy in quantifying the bonding strength in saccharide aqueous solutions.

JOURNAL OF MOLECULAR LIQUIDS (2021)

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Article Cell Biology

Enriched Au nanoclusters with mesoporous silica nanoparticles for improved fluorescence/computed tomography dual-modal imaging

Yifang Yuan et al.

Summary: AuNCs enriched with MSNs can achieve highly sensitive fluorescence/CT dual-modal imaging, indicating potential for disease diagnosis and treatment. The strategy of combining AuNCs with MSNs can provide enhanced imaging capabilities for acquiring more information about diseases.

CELL PROLIFERATION (2021)

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Article Spectroscopy

A fluorescent sensing strategy for ultrasensitive detection of oxytetracycline in milk based on aptamer-magnetic bead conjugate, complementary strand of aptamer and PicoGreen

Amirhossein Bahreyni et al.

Summary: A fluorescent aptasensor was developed for highly sensitive quantification of oxytetracycline in food samples. The method utilizes OTC aptamer conjugated to magnetic beads for sample separation, and the presence of a complementary strand significantly alters the fluorescent signal of the aptasensor, resulting in improved sensitivity. This approach demonstrated great feasibility and potential for analysis of OTC in real samples.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2021)

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Article Food Science & Technology

Advances in flexible surface-enhanced Raman scattering (SERS) substrates for nondestructive food detection: Fundamentals and recent applications

Daorui Zhang et al.

Summary: Flexible SERS substrates, as a promising technology, have great potential in food safety analysis by providing easy sampling and new possibilities for detecting contaminants on food surfaces.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2021)

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Article Food Science & Technology

Magnetic surface-enhanced Raman scattering (MagSERS) biosensors for microbial food safety: Fundamentals and applications

Cuiyun Zhang et al.

Summary: MagSERS platforms, combining magnetic properties and SERS spectra, have shown promising prospects in simplifying, speeding up, and enhancing the sensitivity of biosensors for foodborne biohazards detection. The integration of magnetic capture, separation, and enrichment with SERS substrates, as well as magnetism-mediated hotspot engineering of SERS substrates, is key to the success of MagSERS in food safety analysis.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2021)

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Article Food Science & Technology

Fingerprinting and tagging detection of mycotoxins in agri-food products by surface-enhanced Raman spectroscopy: Principles and recent applications

Zhihui Wu et al.

Summary: SERS is an effective method for rapid and sensitive detection of trace mycotoxins in agri-food products. However, the development of high-performance SERS substrates for on-site quantitative analysis remains a challenging scientific task. Future research should focus on designing reliable substrates, establishing fingerprint libraries, developing accurate chemometric methods, and choosing suitable combined techniques to promote the practical application of SERS in detecting mycotoxin contamination in the agri-food industry.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2021)

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Review Chemistry, Multidisciplinary

Bioinspired Nanomodification Strategies: Moving from Chemical-Based Agrosystems to Sustainable Agriculture

Liang Xu et al.

Summary: While agrochemicals have supported agricultural development, their excessive use poses threats to the environment and human health. Bioinspired synthesis of nanoparticles offers a sustainable and efficient approach, with the potential use of biomass as reducing and capping agents for cost-effective, ecofriendly, and high-performing nanoparticles.

ACS NANO (2021)

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Article Chemistry, Analytical

Reproducible, shelf-stable, and bioaffinity SERS nanotags inspired by multivariate polyphenolic chemistry for bacterial identification

Lunjie Huang et al.

Summary: A multivariate scheme enabled by polyphenolic chemistry was proposed for the green synthesis of metal-phenolic networks (MPNs)-encapsulated silver SERS nanotags, which showed remarkable SERS properties and bacterial affinity. This approach enabled rapid, reproducible, and interference-free SERS detection of target bacteria in food samples, demonstrating the potential of polyphenolic chemistry in food safety detection.

ANALYTICA CHIMICA ACTA (2021)

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Article Chemistry, Applied

Synthesis of bimetallic core-shelled nanoparticles modified by 2-mercaptoethanol as SERS substrates for detecting ferbam and thiabendazole in apple puree

Nisar Hussain et al.

Summary: The study developed a novel SERS substrate by modifying the surface of silver-coated gold nanoparticles with thiol ligands for the rapid assessment of fungicide residues in fruit samples. The results demonstrated the substrate's high detection performance, stability, and potential application in detecting ferbam and thiabendazole residues in apple puree.

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT (2021)

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Article Agriculture, Multidisciplinary

Molecular Characterization and Cross-Allergenicity of Tropomyosin from Freshwater Crustaceans

Panyarat Laurchan et al.

Summary: This study demonstrated that tropomyosin is a major allergen responsible for cross-allergenicity in various shellfish species. Through studying freshwater crustaceans, the role of tropomyosin in the food industry was highlighted.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021)

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Article Chemistry, Multidisciplinary

Synthetic Tunability and Biophysical Basis for Fabricating Highly Fluorescent and Stable DNA Copper Nanoclusters

Neha Tiwari et al.

Summary: The study focused on tuning synthesis variables to create a highly fluorescent and stable DNA copper nanocluster, with optimized concentrations leading to the formation of a <1.0 nm DNA-encapsulated CuNC in AT-DNA. The small size of the AT-DNACuNC enabled discrete electronic transitions and a remarkably strong negative circular dichroism band, correlating with strong fluorescence emission intensity, indicating potential applications in specific DNACuNC detection. Time-dependent fluorescence analysis suggested enhanced photostabilization of these DNACuNCs, with simulation studies highlighting AT-DNA's superiority over GC-DNA in nanocluster formation.

LANGMUIR (2021)

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Article Chemistry, Analytical

A zero-background fluorescent aptasensor for ultrasensitive detection of pesticides based on magnetic three-dimensional DNA walker and poly(T)-templated copper nanoparticles

Yunwei Zhao et al.

Summary: A zero-background fluorescent aptasensor for ultrasensitive detection of pesticides was developed, using CuNPs as a fluorescent signal and a 3-D DNA walker as a signal amplifier. The aptasensor can detect Omethoate with high sensitivity in the linear range of 0-200 nM, providing a facile strategy for pesticide determination in food safety and environmental monitoring.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

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Article Chemistry, Analytical

A fluorescent aptasensor based on copper nanoclusters for optical detection of CD44 exon v10, an important isoform in metastatic breast cancer

Amin Bahmani et al.

Summary: This study aimed to establish an optical aptasensor based on fluorescent metal nanoclusters using v10-specific aptamers for sensitive detection of CD44 v10-enriched cells in breast cancer metastasis. The synthesized nanoclusters showed accurate and high-quality results, with M-Apt4-CuNCs demonstrating the most efficient optical response. The aptasensor had a detection limit of 40 +/- 5 cells per mL.

ANALYTICAL METHODS (2021)

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Article Marine & Freshwater Biology

Visual detection of tropomyosin, a major shrimp allergenic protein using gold nanoparticles (AuNPs)-assisted colorimetric aptasensor

Tushar Ramesh Pavase et al.

Summary: A label-free colorimetric assay using gold nanoparticles was developed to detect the shrimp allergenic protein tropomyosin. The assay showed high efficiency in detecting tropomyosin in water samples and food samples. This method has the potential for easy and efficient visual colorimetric detection of tropomyosin.

MARINE LIFE SCIENCE & TECHNOLOGY (2021)

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Review Chemistry, Multidisciplinary

Introducing reticular chemistry into agrochemistry

Da-Wen Sun et al.

Summary: Agrochemistry is the core engine driving the evolution of modern agriculture, but inefficient use of chemical technologies has led to socio-ecological consequences. Therefore, the development of more productive, precise, and sustainable agricultural tools is needed. Reticular chemistry, especially MOFs and COFs, with their flexible composition, structure, and pore chemistry, can introduce more functionality into the field of agrochemistry.

CHEMICAL SOCIETY REVIEWS (2021)

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Review Chemistry, Multidisciplinary