期刊
FRONTIERS IN MICROBIOLOGY
卷 7, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2016.00400
关键词
giant magnetoresistance; biosensor; magnetic nanoparticle; GMR chip; influenza A virus
类别
资金
- MNDrive: OVPR STEMMA program
- Institute of Engineering in Medicine of the University of Minnesota
- National Science Foundation MRSEC facility program
- Distinguished McKnight University Professorship
- Centennial Chair Professorship
- DROP program from the University of Minnesota
We have developed a simple and sensitive method for the detection of influenza A virus based on giant magnetoresistance (GMR) biosensor. This assay employs monoclonal antibodies to viral nucleoprotein (NP) in combination with magnetic nanoparticles (MNPs). Presence of influenza virus allows the binding of MNPs to the GMR sensor and the binding is proportional to the concentration of virus. Binding of MNPs onto the GMR sensor causes change in the resistance of sensor, which is measured in a real time electrical readout. GMR biosensor detected as low as 1.5 x 10(2) TCID50/mL virus and the signal intensity increased with increasing concentration of virus up to 1.0 x 10(5) TCID50/mL. This study showed that the GMR biosensor assay is relevant for diagnostic application since the virus concentration in nasal samples of influenza virus infected swine was reported to be in the range of 10(3) to 10(5) TCID50/mL.
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