期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 126, 期 -, 页码 459-466出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2014.12.051
关键词
Biopolymer; Nanogels; Methotrexate; pH-sensitivity; Cytotoxicity
资金
- National Natural Science Foundation of China [31371841, 31301426]
- Specialized Research Fund for the Doctoral Program of Higher Education [20130146120019]
- Natural Science Foundation of Hubei Province of China [2013CFB198]
Novel nano-particles were developed from lysozyme-pectin through self-assembly, and the nanogels could be used as a carrier for the antitumor agent, methotrexate (MTX). The nanogels exhibited spherical with diameters about 109 +/- 2 nm and narrow particle size distribution, as well as negative surface charge. Furthermore, the particle size and morphology of the nanogels hardly changed with the incorporation of MTX. The loading capacity of MTX in nanogels could reach 17.58 +/- 0.85%. MTX-loaded nanogels were pH-dependent, accelerated release of MTX at a decreasing pH from 7.4 to 5.3. The MU assay indicated that encapsulated MTX exhibited higher anticancer activity than free MTX. Meanwhile, MTX-loaded nanogels could be effectively endocytosed by HepG2 cells, resulting in enhanced cancer-cell apoptosis comparing to free MTX. It indicated that the nanogels had good biocompatibility and low toxicity. The obtained nanogels had great potential in the development of a new nanocarrier for anti-cancer drug delivery. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据