期刊
FRONTIERS IN MICROBIOLOGY
卷 7, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2016.01681
关键词
antibodies; antimicrobials; ELISA; immune response; phage cocktail; phage therapy; Staphylococcal Infections
类别
资金
- Operational Program Innovative Economy, Priority Axis 1. Research and Development of Modern Technologies, Measure 1.3 Support for RD projects for entrepreneurs carried out by scientific entities, Submeasure 1.3.1 Development projects as project entitled [POIG 01.03.01-02-003/08]
- grant Innovative bacteriophage preparation for the treatment of diabetic foot - National Centre for Research and Development [POIG 01.03.01-02-048/12]
- Wroclaw Center for Biotechnology under the Program the Leading National Research Center (KNOW) - Minister of Science and Higher Education
In this study, we investigated the humoral immune response (through the release of IgG, IgA, and IgM antiphage antibodies) to a staphylococcal phage cocktail in patients undergoing experimental phage therapy at the Phage Therapy Unit, Medical Center of the Ludwik Hirszfeld Institute of Immunology and Experimental Therapy in Wroclaw, Poland. We also evaluated whether occurring antiphage antibodies had neutralizing properties toward applied phages (K rate). Among 20 examined patients receiving the MS-1 phage cocktail orally and/or locally, the majority did not show a noticeably higher level of antiphage antibodies in their sera during phage administration. Even in those individual cases with an increased immune response, mostly by induction of IgG and IgM, the presence of antiphage antibodies did not translate into unsatisfactory clinical results of phage therapy. On the other hand, a negative outcome of the treatment occurred in some patients who showed relatively weak production of antiphage antibodies before and during treatment. This may imply that possible induction of antiphage antibodies is not an obstacle to the implementation of phage therapy and support our assumption that the outcome of the phage treatment does not primarily depend on the appearance of antiphage antibodies in sera of patients during therapy. These conclusions are in line with our previous findings. The confirmation of this thesis is of great interest as regards the efficacy of phage therapy in humans.
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