Label-free competitive time-resolved fluorescent aptasensor for the detection of Pb2+using ssDNA-sensitized fluorescence of Tb3+ions

Lead (Pb2+) is one of the most toxic heavy metals, and its environmental pollution and serious damages is a global concern. Therefore, it is necessary to develop effective sensing methods. This study describes a new strategy for the design of label-free competitive time-resolved fluorescent (TRF) aptasensor for detecting Pb2+ ions. The sensing principle of this aptasensor is the competition between Pb2+ and Tb3+ ions to bind to the guanine/thymine-rich sequence (lead aptamer) and the sensitized luminescence of Tb3+ by this sequence. The developed TRF aptasensor demonstrated a good linear detection range from 2.5 nM to 150 nM and a limit of detection (LOD) of 645 pM. In addition, the proposed TRF aptasensor has a high selectivity towards Pb2+, and it has also been successfully utilized to detect this ion in milk and human serum samples. This TRF aptasensor offers advantages such as short analysis time, simple operation, low cost, being label-free, and surpassing the interference of background fluorescence of biological samples due to its TRF characteristic. In this paper, we propose a Tb3+/guanine/thymine-rich sequence system for designing different aptasensors against diverse targets by applying a proper guanine-rich sequence.

Automated summary

This study presents a new label-free competitive time-resolved fluorescent (TRF) aptasensor for detecting Pb2+ ions. The aptasensor shows a good linear detection range and high selectivity towards Pb2+, and it has been successfully used in milk and human serum samples. The TRF aptasensor offers advantages such as short analysis time, simple operation, and low cost.