期刊
FOOD CONTROL
卷 156, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2023.110112
关键词
Aflatoxin B 1; Fluorescence; Surface-enhanced Raman scattering; Dual-signal; Aptasensor
A dual-signal aptasensor based on surface-enhanced Raman scattering (SERS) and fluorescence has been developed for the detection of aflatoxin B1. The sensor combines SERS and fluorescence signals through the functionalization of nanoparticles and aptamer, allowing for accurate detection of the target substance.
Herein, a surface-enhanced Raman scattering (SERS) and fluorescence dual-signal aptasensor has been developed for aflatoxin B1 (AFB1) detection. Fluorescence signal source CdTe quantum dots were decorated on the outer of silica nanoparticles and further functionalized with AFB1 aptamer. Raman reporter molecules DTNB were decorated on the surface of gold nanoparticles (AuNPs) together with the aptamer complementary chains. The two parts of nanomaterials were combined by the base complementary pairing principle, to form satellite nanostructures with high SERS and low fluorescence signals following LSPR and FRET effect. The addition of AFB1 led to the shedding of AuNPs due to the higher sensitivity of the aptamer towards the target and resulting in recovered fluorescence and decreased SERS signals. The developed method showed a linear relationship in the range of 10-4-103 ng/mL, with detection limit of 0.094 pg/mL. This technology had strong application prospects due to simplicity of operation and high accuracy.
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