期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 136, 期 -, 页码 859-866出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2015.10.031
关键词
Thymol; Microemulsion; Microstructure; Antimicrobial; Antioxidant
资金
- National Natural Science Foundation of China [31471622]
- Zhejiang Province Natural Science Foundation of China [LY14C200010]
- Fuli Institute of Food Science Zhejiang University [KY201401]
Pseudo ternary phase diagrams were constructed to assess the dilutability of thymol microemulsions using non-ionic (Tween 80), cationic (CTAB), and anionic (SDS) surfactants. We successfully constructed a thymol U-type microemulsion system using Tween 80 as surfactant and studied the microstructural transition along the dilution line at a 90/10 surfactant/oil mass ratio, with thymol and ethanol (3:1, w/w) as the oil phase. Differential scanning calorimetry analysis suggested that the microemulsions gradually inverted from the water-in-oil (W/O) (0-20% water) to the bicontinuous (25-35% water), and finally to the oil-in-water (O/W) (40-90% water) microstructures upon dilution, in good agreement with the conductivity measurements, while the theological results indicated the collapse of rod-like micelles followed by formation of spherical micelles in the O/W region. The activities of the U-type thymol microemulsions are structural dependent. The antimicrobial activity against Escherichia coli and Staphylococcus aureus decreased when the microemulsions transformed from W/O to bicontinuous and O/W structures, while the DPPH scavenging activity increased. (C) 2015 Elsevier B.V. All rights reserved.
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