In-situ microbial protein production by using nitrogen extracted from multifunctional bio-electrochemical system

Upgrading of waste nitrogen sources is considered as an important approach to promote sustainable development. In this study, a multifunctional bio-electrochemical system with three chambers was established, innovatively achieving 2.02 g/L in-situ microbial protein (MP) production via hydrogen-oxidizing bacteria (HOB) in the protein chamber (middle chamber), along with over 2.9 L CO2/(Ld) consumption rate. Also, 69% chemical oxygen demand was degraded by electrogenic bacteria in the anode chamber, resulting in the 394.67 J/L electricity generation. Focusing on the NH4+-N migration in the system, the current intensity contributed 4%-9% in the anode and protein chamber, whereas, the negative effect of -6.69% on contribution was shown in the cathode chamber. On the view of kinetics, NH4+-N migration in anode and cathode chambers was fitted well with Levenberg-Marquardt equation (R-2 > 0.92), along with the well-matched results of HOB growth in the protein chamber based on Gompertz model (R-2 > 0.99). Further evaluating MPs produced by HOB, 0.45 g/L essential amino acids was detected, showing the better amino acid profile than fish and soybean. Multifunctional bio-electrochemical system revealed the economic potential of producing 6.69 /m(3) wastewater according to a simplified economic evaluation.

Automated summary

Upgrading waste nitrogen sources is an important approach to promote sustainable development. In this study, a multifunctional bio-electrochemical system was established, achieving high microbial protein production, CO2 consumption, and electricity generation. The migration of NH4+-N in the system followed kinetic models, and the hydrogen-oxidizing bacteria in the protein chamber produced high-quality amino acids.