Preparation and potential antitumor activity of alginate oligosaccharides degraded by alginate lyase from Cobetia marina

It is of great significance to develop marine resources and study its potential biological activity by using alginate lyase produced by marine psychrophilic bacteria. In the previous study, a new marine psychrophilic bacterium (Cobetia marina HQZ08) was screened from the growth area of Laminaria japonica, and it was found that the strain could efficiently produce alginate-degrading enzyme (Aly30). In this paper, the ability of Aly30 to degrade alginate was optimized and the optimal degradation conditions were obtained. It was found that the main degradation product of alginate oligosaccharides was trisaccharide. In vitro cell experiments showed that the antitumor activity of low molecular weight alginate oligosaccharides was better than that of high molecular weight alginate oligosaccharides. In summary, Aly30 had the potential to produce alginate oligosaccharides with low degree of polymerization and antitumor activity, which provided a reference for the enzymatic preparation and application of alginate oligosaccharides.

Automated summary

The use of alginate lyase produced by marine psychrophilic bacteria for the development of marine resources and study of its potential biological activity is of great significance. This study optimized the degradation conditions of alginate by a marine psychrophilic bacterium and found that it can produce low molecular weight alginate oligosaccharides with potential antitumor activity.