期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 253, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2023.127024
关键词
Cytochrome P450; Insecticides resistance; Insecticide metabolism; Transcriptional regulation
Many insect species have developed various resistance mechanisms in response to insecticide selection. This study focuses on understanding the molecular mechanisms of P450-mediated insecticide resistance using the house fly. The results highlight the common involvement of the CncC pathway in adaptive evolution and provide evidence for parallel evolution in P450-mediated insecticide resistance.
Populations of many insect species have evolved a variety of resistance mechanisms in response to insecticide selection. Current knowledge about mutations responsible for insecticide resistance is largely achieved from studies on target-site resistance, while much less is known about metabolic resistance. Although it is well known that P450 monooxygenases are one of the major players involved in insecticide metabolism and resistance, understanding mutation(s) responsible for CYP-mediated resistance has been a big challenge. In this study, we used the house fly to pursue a better understanding of P450 mediated insecticide resistance at the molecular level. Metabolism studies illustrated that CYP6G4 had a broad-spectrum metabolic activity in metabolizing insecticides. Population genotyping revealed that the CYP6G4v1 allele harboring a DNA insertion (MdIS1) had been selected in many house fly populations on different continents. Dual luciferase reporter assays identified that the MdIS1 contained a CncC/Maf binding site, and electrophoretic mobility shift assay confirmed that transcription factor CncC was involved in the MdIS1-mediated regulation. This study highlights the common involvement of the CncC pathway in adaptive evolution, and provides an interesting case supportive of parallel evolution in P450-mediated insecticide resistance in insects.
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