期刊
PHYTOMEDICINE
卷 121, 期 -, 页码 -出版社
ELSEVIER GMBH
DOI: 10.1016/j.phymed.2023.155045
关键词
Tumor; c-Myc; HIF-1 alpha; Lactic acid; Glycolysis; Mitochondrial oxidative phosphorylation
In this study, the researchers found that ISL has anti-gastric cancer activity by inhibiting the growth of gastric cancer and regulating apoptosis-related and metabolism-related protein expression. ISL blocked glucose uptake and suppressed production and secretion of lactic acid, which was accompanied with suppressed mitochondrial oxidative phosphorylation and glycolysis but increased ROS accumulation. Additionally, ISL inhibited the cell viability of gastric cancer by depressing protein expression of c-Myc and HIF-1α, while inducing energy metabolic collapse mediated by PDHK1/PGC-1α.
Background: Isoliquiritigenin (ISL), a natural flavonoid, has anti-tumor activity. But, the understanding of the impact and molecular mechanism of ISL on the growth of gastric cancer (GC) remains limited. Purpose: The study was to explore the tumor suppressive effect of ISL on GC growth both in vitro and in vivo, meanwhile, clarify its molecular mechanisms. Methods: Cell viability was detected by cell counting kit-8 (CCK-8) assay. Apoptotic cells in vitro were monitored by Hoechst 33,342 solution. Protein expression was assessed by Western blot. Reactive oxygen species (ROS) level was evaluated by utilizing 2 ',7 '- dichlorofluorescin diacetate (DCFH-DA). Lactic acid level was detected with L-lactate assay kit. Glucose uptake was monitored with fluorescently tagged glucose 2-[N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl)amino]-2-deoxyglucose (2-NBDG). Glycolytic proton efflux rate (GlycoPER) was evaluated by glycolytic rate assay kit. Oxygen consumption rate (OCR) was conducted by mito stress test kit. A nude mouse model of gastric cancer cell xenograft was established by subcutaneous injection with MGC803 cells. Pathological changes were evaluated by using H&E staining. Cell apoptosis in vivo was evaluated by terminal deoxynucleotide transferase mediated dUTP nick end labeling (TUNEL) assay. Results: ISL remarkably suppressed GC growth and increased cell apoptosis. It regulated apoptosis-related and metabolism-related protein expression both in vitro and in vivo. ISL blocked glucose uptake and suppressed production and secretion of lactic acid, which was accompanied with suppressed mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis but increased ROS accumulation. Overexpression of peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1 alpha), cellular-myelocytomatosis viral oncogene (c-Myc), hypoxia inducible factor-1 alpha (HIF-1 alpha), glucose transporter 4 (GLUT4) or pyruvate dehydrogenase kinase 1 (PDHK1), could abolish ISL-induced inhibition of cell viability in GC cells. Conclusion: These findings implicated that ISL inhibits GC growth by decreasing GLUT4 mediated glucose uptake and inducing PDHK1/PGC-1 alpha-mediated energy metabolic collapse through depressing protein expression of c-Myc and HIF-1 alpha in GC, suggesting its potential application for GC treatment.
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