期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 126, 期 -, 页码 265-272出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2014.12.034
关键词
Polyvinylidene fluoride (PVDF); HAP modified with KH792 (KH792-HAP); Mixed matrix membranes (MMMs); Lysozyme (LZ); Adsorption
资金
- National Natural Science Foundation of China [51203020]
- Scientific Research Starting Foundation for Returned Overseas Chinese Scholars, Ministry of Education of China
- Donghua University Central University Scientific Research Special Fund [2232012D3-32]
The silane coupling agent, N-(beta-aminoethyl)-gamma-aminopropyltrimethoxy silane (KH792), was employed to modify the surfaces of nano-hydroxyapatite (HAP) particles. The mixed matrix membranes (MMMs) were prepared by embedding pure HAP and HAP modified with KH792 (KH792-HAP) inside polyvinylidene fluoride (PVDF) matrix respectively. The MMMs were further characterized concerning permeability and adsorption capacity. Langmuir adsorption isotherm provides better fit for HAP and KH792-HAP than Freundlich isotherm. KH792-HAP has better distribution in the polymeric matrix compared to HAP in the polymeric matrix. The MMMs showed purification of enzyme via static adsorption and dynamic adsorption, and showed the potential of using MMMs for enzyme capturing in enzyme purification techniques. The lysozyme (LZ) was used as a model enzyme. The properties and structures of MMMs prepared by immersion phase separation process were characterized by pure water flux, LZ adsorption and scanning electron microscopy (SEM). (C) 2014 Elsevier B.V. All rights reserved.
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