Rapid and sensitive determination of folic acid by facile synthesized dsDNA-CuNCs fluorescent probe

Folic acid (FA) is one of the essential vitamins for the growth and reproduction of human cells, and it is of great significance to detect FA in real time. In this study, we designed double-stranded DNA (dsDNA)-templated copper nanoclusters (CuNCs) as a nanoprobe for rapid, highly sensitive and selective detection of FA via the inner filter effect (IFE). The dsDNA-CuNCs nanoprobe with strong stability could be prepared facilely in only 5 min at room temperature and testing could be completed in 12.5 min. This method exhibited a satisfactory linear response to FA in the concentration range of 1.25-50.00 mu M and a low limit of detection (LOD) of 0.34 mu M. The dsDNA-CuNCs sensing probe exhibited excellent selectivity and was successfully applied in serum and tablets for FA detection with recoveries of 97.87% to 105.41%. This fluorescence method provides a straightforward label-free biosensor platform for rapid detection of FA, indicating great potential in biological applications for point-of-care testing. Folic acid (FA) is one of the essential vitamins for the growth and reproduction of human cells, and it is of great significance to detect FA in real time.

Automated summary

In this study, a rapid, highly sensitive and selective detection method for folic acid (FA) was developed using a dsDNA-CuNCs nanoprobe. The method exhibited a satisfactory linear response and low detection limit, and was successfully applied in FA detection in serum and tablets.