期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 125, 期 -, 页码 90-95出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2014.11.007
关键词
Carbon dots; Fluorescence; Biosensor; Hydrogen peroxide; Acetylcholinesterase
资金
- National Natural Science Foundation of China [61178035, 61171049, 81171454]
- National Hi-Tech. Research and Development Program of China [2011AA02A114]
- State Key Laboratory of Bioelectronics of Southeast University
The carbon dots (C-dots) with high fluorescence quantum yield were prepared using hydrothermal method. C-dots have been adopted as probes for the fluorescence turn-off detection of H2O2 based on the special sensibility for the hydroxyl radical. And then the biosensors for the detection of substrate and enzymes activities were established in the acetylcholinesterase reaction system, which were related to the production of H2O2. Specifically, the proposed fluorescent biosensor was successfully applied to detect the concentration of choline (in the range from 0.025 to 50 mu M) and acetylcholine (in the range from 0.050 to 50 mu M), and the activity of choline oxidase (in the range from 1 to 75 U/L) and acetylcholinesterase (1 to 80 U/L). These results showed a sensitive, universal, nontoxic and eco-friendly detecting technique has been developed. (C) 2014 Elsevier B.V. All rights reserved.
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