Assessment of Hepatic Lesions After non-Thermal Tumor Ablation by Irreversible Electroporation in a Pig Model

Irreversible electroporation (IRE) is a non-thermal and minimal invasive modality to ablate pathologic lesions such as hepatic tumors. Histological analysis of the initial lesions after IRE can help predict ablation efficacy. We aimed to investigate the histological characteristics of early hepatic lesions after IRE application using animal models. IRE (1500 V/cm, a pulse length of 100 mu s, 60 or 90 pulses) was applied to the liver of miniature pigs. H&E and TUNEL staining were performed and analyzed. Ablated zones of pig liver were discolored and separated from the normal zone after IRE. Histologic characteristics of ablation zones included preserved hepatic lobular architecture with a unique hexagonal-like structure. Apoptotic cells were detected, and sinusoidal dilatation and blood congestion were observed, but hepatic arteries and bile ducts were intact around the ablation zones. The early lesions obtained by delivering monophasic square wave pulses through needle electrodes reflected typical histological changes induced by IRE. Therefore, it was found that the histological assessment of the early hepatic lesion after IRE can be utilized to predict the IRE ablation effect.

Automated summary

This study investigated the histological characteristics of early hepatic lesions after irreversible electroporation (IRE) application in animal models. The results showed that the ablation zones had preserved hepatic lobular architecture with a unique hexagonal-like structure, presence of apoptotic cells, sinusoidal dilatation, and blood congestion. The intact hepatic arteries and bile ducts around the ablation zones indicated that IRE had minimal impact on these structures. Therefore, histological assessment of early hepatic lesions can be utilized to predict the effectiveness of IRE ablation.