期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 134, 期 -, 页码 370-376出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2015.07.014
关键词
Anti-infective; Co-culture; Osteoblast; Implant; Chitosan immobilized titanium
资金
- National Institutes of Health [R21AR065625]
In order to evaluate the anti-infective efficacy of the titanium implant materials, two co-culture systems, a low-bacteria/osteoblast (L-B) and a high-bacteria/osteoblast system (H-B), were established. Untreated (UN-Ti), sulfuric acid-treated (SA-Ti), and chitosan immobilized titanium (SA-CS-Ti) materials were developed and evaluated. Bacteria and osteoblast behaviors, including initial attachment (evaluated at 30 mins), adhesion (evaluated at 4h), and osteoblast spreading on each material surface were evaluated using quantification assays, scanning electron microscopy (SEM), and confocal microscopy. Quantification analysis at 30 mins showed significantly higher number of osteoblast present on SA-CS-Ti in both L-B (10,083 +/- 2626) and H-B (23,592 +/- 2233) than those on the UN-Ti (p <0.05). SEM observation and confocal microscopy results showed more surface area was occupied by adhered osteoblasts on SA-CS-Ti than UN-Ti and SA-Ti in both co-culture systems at 30 mins. At all time points, SA-CS-Ti had the lowest level of bacterial adhesion compared to UN-Ti and SA-Ti in both co-culture systems. A significantly (p <0.05) lower number of bacteria were recovered from SA-CS-Ti (2233 +/- 681) in the H-B system compared to UN-Ti (5367 +/- 1662) and SA-Ti (4533 +/- 680) at 4h. Quantitative and qualitative coculture results show the great potential of chitosan immobilization onto implant materials to prevent implant-associated infections. (C) 2015 Elsevier B.V. All rights reserved.
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