This article presents a protocol for tanycyte-neuron paired whole-cell patch-clamp recording in living mouse brain slices. It provides detailed steps for mice generation, solution preparation, and dissection, as well as the realization of slices and patch-clamp recordings. The protocol can be adapted to study metabolic coupling between tanycytes and any neuronal population.
Here, we present a protocol for tanycyte-neuron paired whole-cell patch-clamp recording in living mouse brain slices. We describe steps for mice generation, solution preparation, and dissection. We then detail realization of slices and patch-clamp recordings. While we use, as an example, tanycytes of the arcuate nucleus of the hypothalamus and pro-opiomelanocortin neurons, this protocol can be adapted to study metabolic coupling between tanycytes and any neuronal population.For complete details on the use and execution of this protocol, please refer to Lhomme et al. (2021).1
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