期刊
ADVANCED SCIENCE
卷 -, 期 -, 页码 -出版社
WILEY
DOI: 10.1002/advs.202303279
关键词
cataract; crowding; crystallins; hydrogel; optics
This study investigates the role and interactions of crystallins in the lens and their impact on transparency. By studying crystallin combinations and properties, it was found that beta-crystallins play a crucial role in maintaining lens transparency. These findings contribute to the design of new transparent materials and enhance understanding of transparency regulation mechanisms.
Positioned within the eye, the lens supports vision by transmitting and focusing light onto the retina. As an adaptive glassy material, the lens is constituted primarily by densely-packed, polydisperse crystallin proteins that organize to resist aggregation and crystallization at high volume fractions, yet the details of how crystallins coordinate with one another to template and maintain this transparent microstructure remain unclear. The role of individual crystallin subtypes (alpha, beta, and gamma) and paired subtype compositions, including how they experience and resist crowding-induced turbidity in solution, is explored using combinations of spectrophotometry, hard-sphere simulations, and surface pressure measurements. After assaying crystallin combinations, beta-crystallins emerged as a principal component in all mixtures that enabled dense fluid-like packing and short-range order necessary for transparency. These findings helped inform the design of lens-like hydrogel systems, which are used to monitor and manipulate the loss of transparency under different crowding conditions. When taken together, the findings illustrate the design and characterization of adaptive materials made from lens proteins that can be used to better understand mechanisms regulating transparency. Human vision is enabled through the transmission, refraction, and color-sensing of light through the eye. Combining analytical and computational methods, the properties and interactions of lens crystallins that enable their organized, dense-packing into a transparent microstructure are explored. image
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