期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 136, 期 -, 页码 545-552出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2015.09.047
关键词
Fusion; Cholesterol; Non-lamellar phases; Thermal fluctuations
资金
- Spanish Ministerio de Ciencia e Innovacion [BFU2011-28566, BFU2012-36241]
- Basque Government [IT-849-13, 11838-13]
Membrane fusion is an important phenomenon in cell biology and pathology. This phenomenon can be modeled using vesicles of defined size and lipid composition. Up to now fusion models typically required the use of chemical (polyethyleneglycol, cations) or enzymatic catalysts (phospholipases). We present here a model of lipid vesicle fusion induced by heat. Large unilamellar vesicles consisting of a phospholipid (dioleoylphosphatidylcholine), cholesterol and diacylglycerol in a 43:57:3 mol ratio were employed. In this simple system, fusion was the result of thermal fluctuations, above 60 degrees C. A similar system containing phospholipid and cholesterol but no diacylglycerol was observed to aggregate at and above 60 degrees C, in the absence of fusion. Vesicle fusion occurred under our experimental conditions only when P-31 NMR and cryo-transmission electron microscopy of the lipid mixtures used in vesicle preparation showed non-lamellar lipid phase formation (hexagonal and cubic). Non-lamellar structures are probably the result of lipid reassembly of the products of individual fusion events, or of fusion intermediates. A temperature-triggered mechanism of lipid reassembly might have occurred at various stages of protocellular evolution. (C) 2015 Published by Elsevier B.V.
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