期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 305, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2023.123497
关键词
Fluoroquinolones; Fluorescent aptamer sensor; Cadmium selenide quantum dots; Graphene oxide
类别
In this work, a novel aptasensor based on the fluorescence quenching property of graphene oxide (GO) and the fluorescence characteristic of cadmium selenide quantum dots (CdSe QDs) was developed for highly sensitive detection of fluoroquinolones contamination. The aptasensor showed reliable detection performance and promising potential for the detection of fluoroquinolones.
In view of the urgent need for fluoroquinolones contamination detection in the fields of food safety, a novel aptasensor based on the fluorescence quenching property of graphene oxide (GO) and the fluorescence char-acteristic of cadmium selenide quantum dots (CdSe QDs) was developed for fluoroquinolones highly sensitive detection in this work. The CdSe QDs with carboxyl-rich surface were synthesized successfully and fluoresced at 525 nm under the optimal excitation light of 366 nm. Based on the hydrophobic and pi-pi stacking between GO and aptamer, aptamer labeled by CdSe QDs fluorescence (CdSe QDs-apt) were adsorbed by GO and the fluorescence of CdSe QDs was quenched. After the aptamer combined specifically with fluoroquinolones, greater specific force lead to the desorption of CdSe QDs-apt from GO and fluorescence recovery. Represented by Ciprofloxacin (CIP), a member of fluoroquinolones, the fluorescence emission increased with the increasing of CIP concentrations from 8 nM to 500 nM, and the detection limit was 0.42 nM. The spiked recoveries in real samples of honey and milk were 91.5-96.9 % and 90.3-95.2 %, respectively, indicating that the aptasensor was reliable. Moreover, the fluorescence responses of multiple members of fluoroquinolones were found to be consistent, denoting that the fluorescence aptasensor can be used to detect the total amount of multiple members of fluoroquinolones. These results showed that the aptasensor can be used as a promising platform for fluo-roquinolones detection.
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