Optimal dietary lysine improves the growth performance, molting frequency, lipid metabolism, immunity and antioxidant of postlarval mud crab Scylla paramamosain

A 56-day feeding trial was designed to investigate the effect of different dietary lysine (Lys) levels on the growth, molting, lipid metabolism, antioxidant, and immunity of postlarva Scylla paramosain. Six diets with 1.20%, 1.46%, 1.84%, 2.06%, 2.35%, and 2.67% Lys levels (dry matter) were formulated and experimented on the postlarva Scylla paramamosain. The results showed that Scylla paramamosain fed with the 2.06% lysine obtained the highest growth performance and the highest molting frequency. Appropriate dietary Lys levels (1.73-2.03%) can improve the antioxidant capacity of Scylla paramamosain. Although unaffected by dietary Lys, the whole -body lipid content of the 1.20% Lys group was numerically higher than those of the 1.46-2.67% Lys groups. The variation in whole-body crude protein content showed a similar trend with those in the content of EAA and NEAAs, with the highest whole-body crude protein content appearing in the 2.06% Lys group. The crabs obtained the highest expression of igf, fas, EcR and fabp genes in the 2.06% Lys group. When dietary Lys levels were > 1.20%, the transcription level of immune related-genes increased. As suggested by the results of the second order polynomial regression analysis of weight gain, the Lys requirement of postlarva Scylla paramamosain is 2.03% (4.51% of dietary protein).

Automated summary

This study investigates the effect of different dietary lysine levels on the growth, molting, lipid metabolism, antioxidant, and immunity of Scylla paramosain postlarva. The results show that the postlarva fed with 2.06% lysine exhibited the highest growth performance and molting frequency. Appropriate lysine levels can improve the antioxidant capacity of Scylla paramosain. The study also found that lipid and protein content were influenced by lysine levels.