Olive mill wastewater treatment using natural adsorbents: phytotoxicity on durum wheat (Triticum turgidum L. var. durum) and white bean (Phaseolus vulgaris L.) seed germination

This research was undertaken to optimize the phenolic compound removal from Olive Mill Wastewater (OMW) by sawdust and red clay as natural adsorbents. Fractional factorial experimental design at 2(5-1) was used in order to optimize the experimental conditions for high removal efficiency. Statistics ANOVA analysis, Fisher's test, and Student's test suggested that the adsorbent dose has the most significant influence on polyphenol removal for both adsorbents. The maximum removal of polyphenols by sawdust reached 49.6% at 60 degrees C by using 60 g/L of adsorbent dose, pH 2, reaction time of 24 h, and agitation speed of 80 rpm. Whereas, for red clay, 48.08% of polyphenols removal was observed under the same conditions for sawdust except the temperature of 25 degrees C instead of 60 degrees C. In addition, the thermodynamic parameters suggested spontaneous process for both adsorbents, endothermic for the sawdust and exothermic for red clay. Furthermore, the phytotoxicity effect of OMW on durum wheat (Triticum turgidum L. var. durum) and white bean (Phaseolus vulgaris L.) seed germination was investigated. The obtained results showed that the untreated OMW inhibited the seed germination of T. turgidum and P. vulgaris seeds. OMW treatment with red clay followed by dilution (95% water) resulted in 87 and 30% germination of P. vulgaris and T. turgidum, respectively. While, the treatment of OMW with sawdust and dilution at 95% resulted in 51 and 26% germination of P. vulgaris and T. turgidum, respectively.

Automated summary

This study aimed to optimize the removal of phenolic compounds from Olive Mill Wastewater (OMW) using sawdust and red clay as natural adsorbents. The experimental conditions were optimized using a fractional factorial experimental design, and the most significant factors affecting polyphenol removal were determined. The results showed that both sawdust and red clay were effective in removing polyphenols from OMW, and the maximum removal was achieved at specific temperature, pH, adsorbent dose, reaction time, and agitation speed. Furthermore, the study also investigated the phytotoxicity effect of untreated OMW on durum wheat and white bean seed germination, and found that treatment with red clay followed by dilution significantly improved the germination rate of both crops compared to treatment with sawdust.