A new difunctional liquid crystal nanosurface molecularly imprinted polyitaconic acid nanoprobe for SERS/RRS determination of ultratrace melamine

In this paper, a new dimode scattering spectral method for rapid detection of ultratrace melamine (ML) in dairy products was established by coupling nanosurface molecular imprinting technology with nanocatalytic amplification reaction of liquid crystal particles. It was found that liquid crystal cholesteryl butyrate (CBU) nanosurface imprinted polymers (CBU@MIP) not only recognized ML but also catalyzed the nano indicator reaction of HAuCl4-sodium formate to produce gold nanoparticles with surface-enhanced Raman scattering (SERS) and resonance Rayleigh scattering (RRS) effect. When ML was added, it specifically combined with CBU@MIP to form CBU@MIP-ML conjugates with strong catalytic activity, and SERS and RRS signals increased linearly with the detection limits of 0.0072 pmol/L and 0.093 pmol/L respectively. The method was applied to the determination of ML in dairy products and plastic tablewares with relative standard deviation (RSD) of 2.2-4.4 % and 1.6-4.7 %, and recovery of 95.4 %-108.3 % and 95.9-108.6 % respectively.

Automated summary

A new method for rapid detection of ultratrace melamine in dairy products was established by coupling nanosurface molecular imprinting technology with nanocatalytic amplification reaction of liquid crystal particles. The method not only recognized melamine, but also catalyzed the production of gold nanoparticles with surface-enhanced Raman scattering and resonance Rayleigh scattering effects. The method showed high accuracy and stability in the determination of melamine in dairy products and plastic tablewares.