4.3 Review

microRNA as biomarkers in tuberculosis: a new emerging molecular diagnostic solution

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Summary: This study characterized exosomes released from THP-1 macrophages cultured in serum-free, ultra-centrifuged medium upon Mycobacterium tuberculosis infection. The results showed that the serum-free medium supported cell growth and allowed for the successful isolation of exosomes expressing CD9, CD63, and CD81 markers, without the presence of Mtb proteins. This suggests that the use of serum-free medium can offer advantages for exosome research and improve extracellular vesicle studies.

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