4.8 Article

High-efficient production of L-homoserine in Escherichia coli through engineering synthetic pathway combined with regulating cell division

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Agricultural Engineering

Dual-channel glycolysis balances cofactor supply for L-homoserine biosynthesis in Corynebacterium glutamicum

Ning Li et al.

Summary: An efficient strain for producing L-homoserine was constructed by enhancing the pentose phosphate pathway (PPP) and introducing the Entner-Doudoroff (ED) pathway. Coexpression of NADH-dependent aspartate-4-semialdehyde dehydrogenase and aspartate dehydrogenase increased the titer from 11.3 to 13.3 g/L. Dual-channel glycolysis, achieved by coexpressing NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase (NADP-GPD) and NAD+-dependent (NAD-GPD), increased the titer by 48.6% to 16.8 g/L. The engineered strain Cg18-1 achieved the highest reported titer of 63.5 g/L L-homoserine in C. glutamicum. This dual-channel glycolysis strategy provides a reference for efficient biosynthesis of other target products through automatic cofactor regulation.

BIORESOURCE TECHNOLOGY (2023)

Article Agricultural Engineering

Multidimensional engineering of Escherichia coli for efficient synthesis of L-tryptophan

Mi Tang et al.

Summary: A riboswitch-based high-throughput screening platform was established to construct a powerful L-tryptophan-producing cell. By regulating gene expression and fine-tuning related modules, the biosynthesis efficiency and yield of L-tryptophan were improved. A novel transcription factor YihL was discovered to negatively regulate L-tryptophan biosynthesis. Dynamic regulation of branch pathways through self-regulated promoters and improved cofactor supply further enhanced L-tryptophan biosynthesis. Without additional supplementation, strain Trp30 achieved a yield of 42.5 g/L and a glucose conversion efficiency of 0.178 g/g after 48 hours of cultivation in a 5-L bioreactor.

BIORESOURCE TECHNOLOGY (2023)

Article Agricultural Engineering

An insight into omics analysis and metabolic pathway engineering of lignin-degrading enzymes for enhanced lignin valorization

Manish Paul et al.

Summary: This review focuses on the research progress of lignin-degrading microorganisms and enzymes associated with ligninolytic activity, which play a crucial role in the breakdown of lignocellulosic biomass. Analyzing multi-omics data highlights the potential value of fungal-produced enzymes in lignocellulosic material degradation, giving them an advantage in their ecological niches.

BIORESOURCE TECHNOLOGY (2023)

Article Agriculture, Multidisciplinary

Module-Guided Metabolic Rewiring for Fucosyllactose Biosynthesis in Engineered Escherichia coli with Lactose De Novo Pathway

Mengli Li et al.

Summary: In this study, an efficient E. coli cell factory was engineered to produce 2'/3-fucosyllactose (2'/3-FL), a beneficial compound for infant health. The strategy involved metabolic network remodeling and resulted in high yields of 2'-FL and 3-FL. This work provides a safe chassis for the production of other lactose-independent human milk oligosaccharides (HMOs) using E. coli as the host.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022)

Article Biotechnology & Applied Microbiology

Development of a nonauxotrophic L-homoserine hyperproducer in Escherichia coli by systems metabolic engineering

Mengmeng Cai et al.

Summary: In this study, a wild-type Escherichia coli was converted to a high-producer of L-homoserine using metabolic engineer strategies. By regulating the degradation pathway, optimizing copy numbers, and modifying the transport system, a final engineered strain was able to efficiently produce a high level of L-homoserine.

METABOLIC ENGINEERING (2022)

Article Agricultural Engineering

Genomics and transcriptomics-guided metabolic engineering Corynebacterium glutamicum for L-arginine production

Zhenqiang Zhao et al.

Summary: L-arginine is an important amino acid used in food and pharmaceutical industries. This study investigated the metabolic mechanisms and fermentation conditions affecting L-arginine production. By generating mutant strains and conducting multi-omic analysis, vital mutations in the L-arginine operon and glutamate synthase were identified. The study also applied systematic metabolic engineering to enhance L-arginine production by modifying key enzymes and increasing cofactor availability. Through fermentation optimization, the engineered strain produced a high yield of L-arginine. This study provides new insights into boosting L-arginine production.

BIORESOURCE TECHNOLOGY (2022)

Article Agricultural Engineering

High-level production of L-valine in Escherichia coli using multi-modular engineering

Yanan Hao et al.

Summary: By using a multi-modular strategy, a high-yielding L-valine-producing strain of Escherichia coli was obtained. Through modifications in the biosynthetic pathway, transport module, transcription factors, and NADPH supply, the strain achieved a high L-valine titer. This study is of significance for enhancing the production performance of L-valine and its derivatives in cell factories.

BIORESOURCE TECHNOLOGY (2022)

Article Biotechnology & Applied Microbiology

Escherichia coli minicells with targeted enzymes as bioreactors for producing toxic compounds

Seung-Jin Kim et al.

Summary: Minicells, formed by aberrant cell division, possess stable metabolism and tolerance, serving as a bioreactor platform with higher production capability and precision compared to bacterial cells.

METABOLIC ENGINEERING (2022)

Article Biotechnology & Applied Microbiology

Metabolic engineering of Escherichia coli W3110 for efficient production of homoserine from glucose

Toan Minh Vo et al.

Summary: An efficient microbial cell factory for the production of homoserine from glucose has been developed through engineering of Escherichia coli W3110. The carbon flux in the glucose metabolism pathway was accelerated and balanced, resulting in high titer, yield, and volumetric productivity of homoserine. The metabolic engineering strategy used in this study can be applied to biosynthesize other amino acids and chemicals derived from aspartic acid.

METABOLIC ENGINEERING (2022)

Article Biotechnology & Applied Microbiology

Flux redistribution of central carbon metabolism for efficient production of l-tryptophan in Escherichia coli

Bo Xiong et al.

Summary: The study demonstrates the successful enhancement of L-tryptophan production in Escherichia coli by redistributing central carbon metabolism to improve PEP and E4P pools. Through a combination of metabolic engineering strategies, the engineered strain achieved a high overall yield of L-tryptophan in batch fermentation.

BIOTECHNOLOGY AND BIOENGINEERING (2021)

Article Agricultural Engineering

Enhancing L-glutamine production in Corynebacterium glutamicum by rational metabolic engineering combined with a two-stage pH control strategy

Qinglan Lv et al.

Summary: The study successfully obtained a high-yielding L-glutamine-producing strain through ARTP mutagenesis and high-throughput screening, followed by using a series of rational metabolic strategies to further increase production. A two-stage pH control strategy was proposed to optimize the inconsistent effects of pH on cell growth and L-glutamine production.

BIORESOURCE TECHNOLOGY (2021)

Review Biotechnology & Applied Microbiology

Metabolic engineering of microbes for monoterpenoid production

Kun Zhu et al.

Summary: Monoterpenoids are important natural products with diverse industrial applications. Current extraction methods from plant sources are not economically viable, and chemical synthesis is not practical due to high energy consumption and pollutant discharge. Microbial biosynthesis offers potential solutions, but improving efficiency remains a key challenge.

BIOTECHNOLOGY ADVANCES (2021)

Review Biochemical Research Methods

Synthetic Biology Toolkits and Metabolic Engineering Applied in Corynebacterium glutamicum for Biomanufacturing

Meng Chai et al.

Summary: Corynebacterium glutamicum is widely used in industrial white biotechnology for producing amino acids, fuels, and value-added chemicals. Synthetic biology strategies have been employed for designing and constructing C. glutamicum cells for biomanufacturing and bioremediation. This study focuses on the design and construction of engineered C. glutamicum strains using genomic modification, metabolic flux optimization, and directed evolution, and discusses the applications of C. glutamicum cell factories and future trends.

ACS SYNTHETIC BIOLOGY (2021)

Article Agricultural Engineering

High-level production of L-homoserine using a non-induced, non-auxotrophic Escherichia coli chassis through metabolic engineering

Yu Zhang et al.

Summary: In this study, a high-efficiency Escherichia coli strain capable of producing 60.1 g/L L-homoserine was constructed, achieving the highest fermentative production efficiency of L-homoserine to date. By dynamically attenuating the L-homoserine degradation pathway and employing systems metabolic engineering strategies, the production yield of L-homoserine was significantly increased. This engineered strain can be further applied for scale-up production of L-homoserine and its derivatives.

BIORESOURCE TECHNOLOGY (2021)

Article Biotechnology & Applied Microbiology

Highly efficient production of L-homoserine in Escherichia coli by engineering a redox balance route

Qingxuan Mu et al.

Summary: This study proposes a novel balanced redox metabolic network strategy for highly efficient production of L-homoserine and its derivative amino acids. By optimizing reactions, deleting competitive pathways, and other measures, a high yield of L-homoserine was achieved in fed-batch fermentation process.

METABOLIC ENGINEERING (2021)

Article Biotechnology & Applied Microbiology

Multiplex Design of the Metabolic Network for Production of L-Homoserine in Escherichia coli

Peng Liu et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2020)

Article Biochemistry & Molecular Biology

Light-based control of metabolic flux through assembly of synthetic organelles

Evan M. Zhao et al.

NATURE CHEMICAL BIOLOGY (2019)

Article Biochemistry & Molecular Biology

Deletion of arcA, iclR, and tdcC in Escherichia coli to improve l-threonine production

Zhixiang Ding et al.

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2019)

Article Biochemistry & Molecular Biology

Asymmetric biosynthesis of L-phosphinothricin by a novel transaminase from Pseudomonas fluorescens ZJB09-108

Li-Qun Jin et al.

PROCESS BIOCHEMISTRY (2019)

Review Biotechnology & Applied Microbiology

Impacts of environmental conditions on product formation and morphology of Yarrowia lipolytica

Asma Timoumi et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2018)

Article Biotechnology & Applied Microbiology

Rational modification of tricarboxylic acid cycle for improving L-lysine production in Corynebacterium glutamicum

Jian-Zhong Xu et al.

MICROBIAL CELL FACTORIES (2018)

Review Biotechnology & Applied Microbiology

Solvent Tolerance in Bacteria: Fulfilling the Promise of the Biotech Era?

Hadiastri Kusumawardhani et al.

TRENDS IN BIOTECHNOLOGY (2018)

Review Biochemistry & Molecular Biology

Assembly and activation of the Escherichia coli divisome

Shishen Du et al.

MOLECULAR MICROBIOLOGY (2017)

Article Agricultural Engineering

Production of amino acids - Genetic and metabolic engineering approaches

Jin-Ho Lee et al.

BIORESOURCE TECHNOLOGY (2017)

Review Biotechnology & Applied Microbiology

Morphology engineering of bacteria for bio-production

Xiao-Ran Jiang et al.

BIOTECHNOLOGY ADVANCES (2016)

Article Biochemistry & Molecular Biology

Metabolic engineering of Escherichia coli W3110 for L-homoserine production

Hua Li et al.

PROCESS BIOCHEMISTRY (2016)

Article Biotechnology & Applied Microbiology

Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing

Yifan Li et al.

METABOLIC ENGINEERING (2015)

Article Biotechnology & Applied Microbiology

Engineering the bacterial shapes for enhanced inclusion bodies accumulation

Xiao-Ran Jiang et al.

METABOLIC ENGINEERING (2015)

Article Biotechnology & Applied Microbiology

O-Succinyl-L-homoserine-based C4-chemical production: succinic acid, homoserine lactone, γ-butyrolactone, γ-butyrolactone derivatives, and 1,4-butanediol

Kuk-Ki Hong et al.

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2014)

Article Biotechnology & Applied Microbiology

Combinatorial modulation of galP and glk gene expression for improved alternative glucose utilization

Jiao Lu et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2012)

Article Biochemistry & Molecular Biology

Systems metabolic engineering of Escherichia coli for L-threonine production

Kwang Ho Lee et al.

MOLECULAR SYSTEMS BIOLOGY (2007)

Article Biotechnology & Applied Microbiology

Expression of an anaplerotic enzyme, pyruvate carboxylase, improves recombinant protein production in Escherichia coli

JC March et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2002)

Article Biotechnology & Applied Microbiology

A novel methodology employing Corynebacterium glutamicum genome information to generate a new L-lysine-producing mutant

J Ohnishi et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2002)