期刊
BIOTECHNOLOGY JOURNAL
卷 11, 期 11, 页码 1452-1460出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201600087
关键词
Hepatocytes; Horseradish peroxidase; Poly(ethylene glycol); Redox-responsive hydrogel; Spheroids
资金
- Grants-in-Aid for Scientific Research [16H06369, 15K14238] Funding Source: KAKEN
Cellular spheroids have been received much attention in the biological and biomedical fields, especially as a base material for drug assays, regenerative medicine, and tissue engineering. Hydrogels have potential for scalable preparation of spheroids because they provide a spatial environment suitable for three-dimensional cell cultivation. Herein, the potential use of a redox-responsive hydrogel as a scaffold for preparation and recovery of spheroids is reported. A hydrogel composed of poly(ethylene glycol) (PEG), which can be degraded using cysteine as a reducing agent under mild conditions, is prepared by mixing an octa-thiolated PEG derivative (8-arm PEG-SH), horseradish peroxidase and a small phenolic compound (Glycyl-L-tyrosine). Human hepatocellular carcinoma cells (HepG2) are encapsulated in the hydrogel and cellular spheroids formed by proliferation within the scaffolds. After seven days of cultivation, the size of the HepG2 spheroids reached a diameter between approximate to 40 and 60 mu m, depending on the 8-arm PEG-SH concentration. Liver-specific functions of the HepG2 spheroids such as albumin secretion and urea production are retained at higher levels than those of cells prepared from traditional two-dimensional monolayers. These results suggest that the system presented here has potential for preparation of cellular spheroids for tissue engineering applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据