期刊
BIOMEDICINE & PHARMACOTHERAPY
卷 168, 期 -, 页码 -出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2023.115814
关键词
Colorectal cancer; Hepsin; Suramin; Tumor invasion; Cancer -associated thrombosis
The research group identified a novel hepsin inhibitor, suramin, which can reduce invasion and thrombin generation of colorectal cancer cells. This study suggests the potential of repurposing suramin and its derivatives to enhance molecular targeted therapies against colorectal cancer.
Recently, our group identified serine-protease hepsin from primary tumor as a biomarker of metastasis and thrombosis in patients with localized colorectal cancer. We described hepsin promotes invasion and thrombin generation of colorectal cancer cells in vitro and in vivo and identified venetoclax as a hepsin inhibitor that suppresses these effects. Now, we aspire to identify additional hepsin inhibitors, aiming to broaden the thera-peutic choices for targeted intervention in colorectal cancer.Methods: We developed a virtual screening based on molecular docking between the hepsin active site and 2000 compounds from DrugBank. The most promising drug was validated in a hepsin activity assay. Subse-quently, we measured the hepsin inhibitor effect on colorectal cancer cells with basal or overexpression of hepsin via wound-healing, gelatin matrix invasion, and plasma thrombin generation assays. Finally, a zebrafish model determined whether hepsin inhibition reduced the invasion of colorectal cancer cells over -expressing hepsin.Results: Suramin was the most potent hepsin inhibitor (docking score:-11.9691 Kcal/mol), with an IC50 of 0.66 mu M. In Caco-2 cells with basal or overexpression of hepsin, suramin decreased migration and significantly reduced invasion and thrombin generation. Suramin did not reduce the thrombotic phenotype in the hepsin-negative colorectal cancer cells HCT-116 and DLD-1. Finally, suramin significantly reduced the in vivo inva-sion of Caco-2 cells overexpressing hepsin. Conclusion: Suramin is a novel hepsin inhibitor that reduces its protumorigenic and prothrombotic effects in colorectal cancer cells. This suggests the possibility of repurposing suramin and its derivatives to augment the repertoire of molecular targeted therapies against colorectal cancer.
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