Multi-omics analysis to reveal key pathways involved in low C/N ratio stress response in Pseudomonas sp. LW60 with superior nitrogen removal efficiency

In practical engineering, nitrogen removal at low temperatures or low C/N ratios is difficult. Although strains can remove nitrogen well at low temperatures, there is no research on the performance and deep mechanism of strains under low C/N ratio stress. In this study, Pseudomonas sp. LW60 with superior nitrogen removal efficiency under low C/N ratio stress was isolated at 4 degrees C. With a C/N ratio of 2-10, the NH4+-N removal efficiency was 40.02 %-100 % at 4 degrees C. Furthermore, the resistance mechanism of Pseudomonas sp. LW60 to low C/N ratio stress was deeply investigated by multi-omics. The results of transcriptome, proteome, and metabolome revealed that the resistance of strain LW60 to low C/N ratio stress was attributed to enhanced central carbon metabolism, amino acid metabolism, and ABC transporters, rather than nitrogen removal pathways. This study isolated a strain with low C/N ratio tolerance and deeply explored its tolerance mechanism by multi-omics.

Automated summary

In practical engineering, nitrogen removal becomes difficult at low temperatures or low C/N ratios. This study successfully isolated a strain of Pseudomonas sp. with high nitrogen removal efficiency under low C/N ratio stress and investigated its resistance mechanism in depth.